Heat-treatment method for producing fatty acid-bound alpha-lactalbumin that induces tumor cell death
Heat-treatment method for producing fatty acid-bound alpha-lactalbumin that induces tumor cell death
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DOI：
10.1016/j.bbrc.2008.08.127
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发表时间：
2008-11-07
影响因子：
3.1
通讯作者：
Demura, Makoto
中科院分区：
文献类型：
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作者：
Kamijima, Tatsuro;Ohmura, Ayaka;Demura, Makoto
HAMLET（人类 α-乳清蛋白对肿瘤细胞产生致死作用）在人母乳中被鉴定为 α-乳清蛋白 (LA)-油酸复合物，可选择性杀死肿瘤细胞。尽管它可能具有作为针对各种肿瘤细胞的治疗剂的潜力，但仅存在小批量的生产方法。在本研究中，通过热处理以简单的方法从 LA 和油酸生产复合物。就人类 LA 和油酸而言，热处理样品明显表现出比室温处理样品更强的活性，细胞毒性与 HAMLET 相同。此外，圆二色光谱显示热处理样品失去了三级结构，表明熔球为油酸结合的 LA。 BLA 样品在热处理后也显示出很强的活性。通过热处理批量生产可以有效地将LAs转化为抗肿瘤复合物。 (C) 2008 Elsevier Inc. 保留所有权利。
HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells), which was identified in human breast milk as an alpha-lactalbumin (LA)-oleic acid complex, kills tumor cells, selectively. Although it may have potential as a therapeutic agent against various tumor cells, only low-volume methods for its production exist. In this study, heat treatment was used to produce complexes from LAs and oleic acid using a simple method. In the case of human LA and oleic acid, heat-treated samples apparently showed much stronger activities than those treated at room temperature, with cytotoxicities equal to that of HAMLET. Furthermore, Circular dichroism spectroscopy revealed that heat-treated samples lost their tertiary structure, suggesting a molten globule as oleic acid-bound LA. BLA samples also showed strong activities by heat treatment. Batch production with heat treatment can efficiently convert LAs into tumoricidal complexes. (C) 2008 Elsevier Inc. All rights reserved.