Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver.
Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver.
复制标题
日本血吸虫感染C57BL/6小鼠肝脏IL-9的特性
DOI：
10.1038/s41598-017-02422-8
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发表时间：
2017-05-24
影响因子：
4.6
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Li L;Xie H;Wang M;Qu J;Cha H;Yang Q;Feng Y;Qi Y;Qiu H;Dong N;Huang J
肝肉芽肿性炎症和肝纤维化是日本血吸虫病的主要病理改变。japonicum）感染。本实验研究了白细胞介素-9（IL-9）在肝组织中的表达特性。日本血吸虫感染C57 BL/6小鼠。免疫荧光、qRT-PCR和ELISA检测结果显示，感染后IL-9的表达显著增加（P < 0.01）。流式细胞仪检测结果显示，除Th 9细胞、CD 8 + Tc细胞、NKT细胞和γδT细胞能分泌IL-9外，IL-9+ Th 9细胞的高峰出现在感染早期（第5周）。虽然IL-9中和对肝肉芽肿性炎症的作用有限，但可降低感染小鼠血清中纤维化相关因子PC-Ⅲ的水平（P < 0.05）。综上所述，我们的结果表明IL-9是参与S.日本血吸虫感染引起的肝损害。
Liver granulomatous inflammation and fibrosis were the primary pathological changes observed during Schistosoma japonicum (S. japonicum) infection. In the present study, the characteristics of IL-9 were investigated in the liver of S. japonicum infection C57BL/6 mice. Immunofluorescence, qRT-PCR, and ELISA results demonstrated that the expression of IL-9 significantly increased after infection (P < 0.01). FACS results indicated that the peak of IL-9+ Th9 cells in the liver mononuclear cells appeared at the early phase of infection (week 5), except that Th9 cells, CD8+ Tc cells, NKT and γδT cells could secrete IL-9 in this model. Although IL-9 neutralization has a limited effect on liver granulomatous inflammation, it could decrease the level of fibrosis-associated factor, PC-III, in the serum of infected mice (P < 0.05). Taken together, our results indicated that IL-9 was an important type of cytokine involved in the progression of S. japonicum infection-induced hepatic damage.