FSL-1 Induces MMP-9 Production through TLR-2 and NF-κB/AP-1 Signaling Pathways in Monocytic THP-1 Cells
FSL-1 Induces MMP-9 Production through TLR-2 and NF-κB/AP-1 Signaling Pathways in Monocytic THP-1 Cells
复制标题
DOI：
10.1159/000366310
复制
发表时间：
2014-01-01
影响因子：
--
通讯作者：
Behbehani, Kazem
中科院分区：
文献类型：
--
作者：
Ahmad, Rasheed;Shihab, Puthiyaveetil Kochumon;Behbehani, Kazem
背景：基质金属蛋白酶-9（MMP-9）参与多种炎症性疾病的发病机制。FSL-1（成纤维细胞刺激脂肽-1）诱导单核细胞/巨噬细胞产生细胞因子。然而，目前还不清楚FSL-1是否也能够诱导MMP-9的产生。在此，我们确定了FSL-1是否可以诱导MMP-9的产生，如果是这样的话，涉及哪些信号转导途径。方法：采用实时qPCR和ELISA检测MMP-9的表达。通过使用THP 1-XBlue（TM）细胞、THP 1-XBlue（TM）-defMyD细胞、抗TLR 2 mAb和药理学抑制剂研究信号通路。蛋白质印迹法测定磷酸化和总蛋白。结果：FSL-1诱导MMP-9表达（P
Background: Matrix metalloproteinase-9 (MMP-9) is known to be implicated in the pathogenesis of many inflammatory disorders. FSL-1 (fibroblast-stimulating lipopeptide-1) induces cytokine production by monocytes/macrophages. However, it is unclear whether FSL-1 is also able to induce MMP-9 production. Herein, we determined whether FSL-1 could induce MMP-9 production, and if so, which signal transduction pathway(s) were involved. Methods: MMP-9 expression was assessed with real-time qPCR and ELISA. Signaling pathways were studied by using THP1-XBluer (TM) cells, THP1-XBlue (TM)-defMyD cells, anti-TLR2 mAb and pharmacological inhibitors. Phospho and total proteins were determined by Western blotting. Results: FSL-1 induces MMP-9 expression (P