A quantitative method for staging mouse embryos based on limb morphometry
A quantitative method for staging mouse embryos based on limb morphometry
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DOI：
10.1242/dev.154856
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发表时间：
2018-04-01
期刊：
影响因子：
4.6
通讯作者：
Sharpe, James
中科院分区：
文献类型：
--
作者：
Musy, Marco;Flaherty, Kevin;Sharpe, James
为了确定胚胎小鼠的发育阶段，我们应用几何形态测量方法来改变小鼠肢芽的形状，因为它从胚胎第10天到胚胎第15天后的概念。由于个体发育序列的结果在从头出现的形状特征不存在于早期阶段，我们已经创建了一个标准的个体发育轨迹肢芽发展-在肢体形态发生的形状变化的定量表征。这种形态随时间变化的轨迹也给了我们相反的功能：从形态推断发育阶段的能力，典型的不确定性为2小时。我们介绍eMOSS（胚胎小鼠个体发育分期系统）作为一个快速，可靠，方便和免费提供的在线工具，分期胚胎从二维图像的肢芽，并说明其使用表型早期肢体异常。
To determine the developmental stage of embryonic mice, we apply a geometric morphometric approach to the changing shape of the mouse limb bud as it grows from embryonic day 10 to embryonic day 15 post-conception. As the ontogenetic sequence results in the de novo emergence of shape features not present in the early stages, we have created a standard ontogenetic trajectory for limb bud development - a quantitative characterization of shape change during limb morphogenesis. This trajectory of form as a function of time also gives us the reverse function: the ability to infer developmental stage from form, with a typical uncertainty of 2 h. We introduce eMOSS (embryonic mouse ontogenetic staging system) as a fast, reliable, convenient and freely available online tool for staging embryos from two-dimensional images of their limb buds, and illustrate its use in phenotyping early limb abnormalities.