Postsynaptic CPG15 promotes synaptic maturation and presynaptic axon arbor elaboration in vivo
Postsynaptic CPG15 promotes synaptic maturation and presynaptic axon arbor elaboration in vivo
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DOI：
10.1038/79823
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发表时间：
2000-10-01
影响因子：
25
通讯作者：
Cline, HT
中科院分区：
文献类型：
--
作者：
Cantallops, I;Haas, K;Cline, HT
中枢神经系统回路的形成以神经元结构和突触功能的协调发展为特征。活性调节候选可塑性基因15(Cpg15)编码一种糖基磷脂酰肌醇(GPI)连接的蛋白，其在体内的表达可促进非洲爪哇视顶盖细胞的树突状突起生长。我们现在证明，顶盖细胞cpg15的表达通过降低分支回缩速度显著增加突触前视网膜轴突的形成。来自视顶盖神经元的全细胞记录表明，cpg15的表达通过将功能性AMPA受体招募到突触来促进视网膜顶盖突触的成熟。缺少GPI锚点的截短cpg15的表达不能促进轴突的生长和阻止突触的成熟。这些结果表明，cpg15协同增加突触前、后结构的生长及其突触接触的数量和强度。
The formation of CNS circuits is characterized by the coordinated development of neuronal structure and synaptic function. The activity-regulated candidate plasticity gene 15 (cpg15) encodes a glycosylphosphatidylinositol (GPI)-linked protein whose in vivo expression increases the dendritic arbor growth rate of Xenopus optic tectal cells. We now demonstrate that tectal cell expression of CPG15 significantly increases the elaboration of presynaptic retinal axons by decreasing rates of branch retractions. Whole-cell recordings from optic tectal neurons indicate that CPG15 expression promotes retinotectal synapse maturation by recruiting functional AMPA receptors to synapses. Expression of truncated CPG15, lacking its GPI anchor, does not promote axon arbor growth and blocks synaptic maturation. These results suggest that CPG15 coordinately increases the growth of pre- and postsynaptic structures and the number and strength of their synaptic contacts.