MONOGLYCERIDE PATHWAY OF FAT ABSORPTION ON MAN
MONOGLYCERIDE PATHWAY OF FAT ABSORPTION ON MAN
复制标题
DOI：
10.1172/jci105660
复制
发表时间：
1967-01-01
影响因子：
15.9
通讯作者：
MATTSON, FH
中科院分区：
文献类型：
--
作者：
KAYDEN, HJ;SENIOR, JR;MATTSON, FH
本文研究了5例男性受试者，通过胃或十二指肠插管，给予双标记单甘油三酯或甘油三酯以及标记游离甘油或标记游离油酸，在颈部胸导管插管。甘油标记（氚）和脂肪酸标记（14c）的总回收率分别为35%至53%和35%至57%。即使与胆盐、脂肪酸和单甘油酯混合给药，淋巴脂中放射性游离甘油的回收率仅为4.1%。淋巴甘油酯的两种成分（甘油和脂肪酸）的同位素比值与原始膳食甘油酯的同位素比值的比较表明，在脂肪吸收的初始阶段，这些双标记的单甘油酯几乎没有变化，这表明双标记的单甘油酯完整地进入了淋巴。在主要脂肪吸收期的后期，由于甘油代表的丧失，淋巴脂质比例发生变化，表明单甘油水解和门静脉游离甘油转移。通过分析淋巴甘油三酯中标记脂肪酸的数量和位置，确认了2-单甘油三酯在吸收过程中的完整性质。在脂肪吸收的最初阶段，淋巴甘油三酯各个位置的标记脂肪酸百分比几乎与膳食相同，然后随着脂肪酸的异构化和甘油的完全水解而发生变化。2-单甘油三酯途径似乎是人体正常消化和膳食甘油三酯吸收过程中脂肪吸收的主要途径。
The absorption of fat was studied in 5 male subjects with cannulation of the thoracic duct in the neck by the administration of doubly labeled monoglycerides, or triglyceride as well as labeled free glycerol or labeled free oleic acid, by gastric or duodenal intubation. Total recoveries of the administered glyceride radioactivity from the lymph lipids ranged from 35 to 53% for the glycerol label (tritium) and from 35 to 57% for the fatty acid label (14c). The recovery of administered radioactive free glycerol in lymph lipids was only 4.1%, even when given in mixture with bile salts, fatty acid, and monoglyceride. A comparison of the isotope ratios of the 2 components (glycerol and fatty acid) of the lymph glycerides with the ratios of these components of the original meal glyceride showed little change during the initial period of fat absorption, indicating that the doubly labeled monoglycerides passed into the lymph intact. During the later part of the period of major fat absorption, the ratios in lymph lipids changed due to loss of glycerol representation, indicating monoglyceride hydrolysis and portal venous diversion of free glycerol. Confirmation of the intact nature of 2-monoglyceride during absorption was made by analyzing the amount and position of the labeled fatty acid in the lymph triglycerides. The percentage of labeled fatty acid in the various positions of the lymph triglycerides was virtually identical with that of the meal during the initial period of fat absorption and then changed reflecting isomerization of fatty acids and subsequent complete hydrolysis of the glycerides. The 2-monoglyceride pathway appears to be the major route of fat absorption for man during normal digestion and absorption of dietary triglyceride.