Novel approach to regeneration of periodontal tissues based on in situ tissue engineering:: Effects of controlled release of basic fibroblast growth factor from a sandwich membrane
Novel approach to regeneration of periodontal tissues based on in situ tissue engineering:: Effects of controlled release of basic fibroblast growth factor from a sandwich membrane
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DOI：
10.1089/107632703762687636
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发表时间：
2003-02-01
影响因子：
--
通讯作者：
Shimizu, Y
中科院分区：
文献类型：
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作者：
Nakahara, T;Nakamura, T;Shimizu, Y
为了再生牙周组织，根据“原位组织工程”的新概念，研制了一种由胶原海绵支架和含有碱性成纤维细胞生长因子(BFGF)的明胶微球组成的控制释放系统的夹心膜。在9只Beagle犬的上颌骨和下颌骨的近中无牙区制作了一个3×4×4 mm的三壁牙槽骨缺损区。每组18例，分别植入含或不含碱性成纤维细胞生长因子的夹层膜(100mug)。在第1、2和4周，对3只狗进行组织学评估和组织计量学分析。在整个4周内，血管形成和成骨仅在碱性成纤维细胞生长因子治疗组活跃(p<；0.01)。术后4周裸露的牙根表面形成新的牙骨质(2.4+/-0.9 mm)，再生的胶原纤维垂直排列，部分反映了牙周膜功能的恢复。对照组上皮细胞生长减退，牙根吸收，缺损处充填结缔组织。因此，我们的三明治膜在短时间内成功地诱导了牙周组织的再生。
To regenerate periodontal tissues, a sandwich membrane composed of a collagen sponge scaffold and gelatin microspheres containing basic fibroblast growth factor (bFGF) in a controlled-release system was developed according to the new concept of "in situ tissue engineering." A three-walled alveolar bone defect (3 x 4 x 4 mm) was made bilaterally in edentulous regions created mesially to the canines in both the maxilla and mandible of nine beagle dogs. A sandwich membrane with or without bFGF (100 mug) was implanted in each defect (each group, n = 18). During weeks 1, 2, and 4, histologic evaluation and histometric analyses were performed on three dogs. Throughout the 4 weeks, vascularization and osteogenesis were active only in the bFGF-treated group (p< 0.01). New cementum was formed (2.4 +/- 0.9 mm) on the exposed root surface at 4 weeks, and functional recovery of the periodontal ligament was indicated in part by the perpendicular orientation of regenerated collagen fibers. In the control group, epithelial downgrowth and root resorption occurred and the defects were filled with connective tissue. Thus, our sandwich membrane induced successful regeneration of the periodontal tissues in a short period of time.