Reduction of Inflammatory Responses and Enhancement of Extracellular Matrix Formation by Vanillin-Incorporated Poly(Lactic-co-Glycolic Acid) Scaffolds
Reduction of Inflammatory Responses and Enhancement of Extracellular Matrix Formation by Vanillin-Incorporated Poly(Lactic-co-Glycolic Acid) Scaffolds
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DOI：
10.1089/ten.tea.2012.0001
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发表时间：
2012-10-01
影响因子：
4.1
通讯作者：
Lee, Dongwon
中科院分区：
文献类型：
--
作者：
Lee, Yujung;Kwon, Jeongil;Lee, Dongwon
香草素是香草的主要成分之一，是一种常用的调味剂和防腐剂，具有强大的抗氧化和抗炎活性。本研究通过制备香兰素-聚乳酸-羟基乙酸（PLGA）膜和支架，研究了香兰素对体外和体内炎症反应和细胞外基质（ECM）形成的影响。与纯PLGA膜相比，香草醛掺入PLGA膜诱导了亲水性，导致了更高的细胞附着和增殖。在纯PLGA膜上培养的细胞中，香兰素还能减少活性氧（ROS）的产生，并在体内显著抑制PLGA诱导的炎症反应，炎症细胞的积累减少，纤维囊变薄。采用纤维环（AF）细胞种子多孔PLGA/香兰素支架，评价了香兰素对ECM形成的影响。PLGA/香草醛支架比纯PLGA支架诱导了更多的糖胺聚糖和胶原蛋白的产生，且呈浓度依赖性。基于低水平的炎症反应和增强的ECM形成，香草素掺入的PLGA结构在未来的生物医学应用中有很大的前景。
Vanillin is one of the major components of vanilla, a commonly used flavoring agent and preservative and is known to exert potent antioxidant and anti-inflammatory activities. In this work, vanillin-incorporated poly(lactic-co-glycolic acid) (PLGA) films and scaffolds were fabricated to evaluate the effects of vanillin on the inflammatory responses and extracellular matrix (ECM) formation in vitro and in vivo. The incorporation of vanillin to PLGA films induced hydrophilic nature, resulting in the higher cell attachment and proliferation than the pure PLGA film. Vanillin also reduced the generation of reactive oxygen species (ROS) in cells cultured on the pure PLGA film and significantly inhibited the PLGA-induced inflammatory responses in vivo, evidenced by the reduced accumulation of inflammatory cells and thinner fibrous capsules. The effects of vanillin on the ECM formation were evaluated using annulus fibrous (AF) cell-seeded porous PLGA/vanillin scaffolds. PLGA/vanillin scaffolds elicited the more production of glycosaminoglycan and collagen than the pure PLGA scaffold, in a concentration-dependent manner. Based on the low level of inflammatory responses and enhanced ECM formation, vanillin-incorporated PLGA constructs make them promising candidates in the future biomedical applications.