Transcriptome profiling of runner formation induced by exogenous gibberellin in Fragaria vesca
Transcriptome profiling of runner formation induced by exogenous gibberellin in Fragaria vesca
复制标题
外源赤霉素诱导草莓转跑形成的转录组分析
DOI：
10.1016/j.scienta.2021.109966
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发表时间：
2021-01-29
影响因子：
4.3
通讯作者：
Zhang, Zhihong
中科院分区：
文献类型：
--
作者：
Li, Weijia;Wang, Baotian;Zhang, Zhihong
To acquire more findings on the molecular mechanism of exogenous gibberellin on the growth and living process of strawberry and look into such a mechanism's impacts, the seedlings of Fragaria vesca accession 'Ruegen', a non-runner genotype, were sprayed with different concentrations (0-100 mg/L) exogenous gibberellin (GA(3)). When the concentration of GA(3) reached 50 mg/L, the seedlings produced runners. Illumina HiSeq high-throughput sequencing was performed on the stem apices of strawberry seedlings treated with GA(3) (100 mg/L) and the control, and then, the transcriptomes were compared. RNA-Seq data revealed that many genes related to the synthesis and metabolism of plant hormones and signal transduction showed significant up-regulated or down-regulated expressions in the stem apices treated with GA(3), and the altered expression levels were verified by the quantitative RT-PCR testing approach. Regarding these, this study hereby highlights a crucial insight into the mechanism of gibberellin, and its embedded regulation mechanism pertaining to strawberry-runner formation.
