Immunomodulatory Activity in Vitro and in Vivo of Verbascose from Mung Beans (Phaseolus aureus)
Immunomodulatory Activity in Vitro and in Vivo of Verbascose from Mung Beans (Phaseolus aureus)
复制标题
绿豆（Phaseolus aureus）毛蕊花糖的体外和体内免疫调节活性
DOI：
10.1021/jf503510h
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发表时间：
2014-11-05
影响因子：
6.1
通讯作者：
Zeng, Xiaoxiong
中科院分区：
文献类型：
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作者：
Dai, Zhuqing;Su, Di;Zeng, Xiaoxiong
本研究采用体外细胞模型和体内动物模型研究了绿豆毛蕊花糖的免疫刺激活性。体外实验结果表明，毛蕊花糖能增强腹腔巨噬细胞吞噬中性红的能力，促进一氧化氮和免疫反应分子白细胞介素（IL）-6、IL-1 β、干扰素（IFN）-α、IFN-γ的释放。用200 μ g/mL的毛蕊花糖处理表现出最佳效果。在体内试验中，毛蕊花糖90 mg/kg体重中剂量给药能显著提高免疫抑制小鼠迟发型超敏反应（DTH）的脾指数、脾和血清溶菌酶活性、血清溶血素水平和耳廓肿胀率。结果表明毛蕊花糖具有较强的免疫刺激活性，可作为一种潜在的天然免疫调节剂应用于功能性食品中。
In the present study, the immunostimulatory activity of verbascose from mung beans (Phaseolus aureus) was evaluated by using in vitro cell models and in vivo animal models. The results of in vitro experiments showed that verbascose could enhance the ability of devouring neutral red of peritoneal macrophages and promote the release of nitric oxide and immune reactive molecules such as interleukin (IL)-6, IL-1 beta, interferon (IFN)-alpha, and IFN-gamma. Treatment with verbascose at a dose of 200 mu g/mL exhibited the best effects. For assay in vivo, administration of verbascose at a medium dose of 90 mg/kg body weight could significantly increase the index of spleen, activity of lysozyme in spleen and serum, hemolysin level in serum, and swelling rate of earlap in the delayed type of hypersensitivity (DTH) of immunosuppressed mice. All of the results suggested that verbascose had potent immunostimulatory activity and could be explored as a potential natural immunomodulatory agent in functional foods.