Hydroxyurea enhances post-fusion hyphal extension during sexual development in C. neoformans var. grubii.
Hydroxyurea enhances post-fusion hyphal extension during sexual development in C. neoformans var. grubii.
复制标题
羟基脲增强 C. neoformans var. 有性发育过程中融合后菌丝的延伸。
DOI：
10.1007/s11046-011-9474-y
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发表时间：
2012
期刊：
影响因子：
5.5
通讯作者：
Panepinto,JohnC
中科院分区：
文献类型：
--
作者：
Zulkifli,MNaim;Kaur,JanNaseer;Panepinto,JohnC
交配与性发展公司。H99背景的新形式变种通常不如实验室产生的同源C那么健壮。JEC21背景中的新生变种新生杆菌。在白色念珠菌和丝状酵母菌中，DNA合成减慢和复制应激反应的参与，如羟基尿素(HU)处理，分别诱导丝状化和伪菌丝生长。在本研究中，我们探讨了HU治疗对NC的影响。新形态变种，新变种。无论是在液体培养上还是在固体YPD或V8琼脂上，HU处理都不能诱导酵母细胞丝状生长。在相反的交配伙伴的存在下，我们观察到在Hu的存在下菌丝的早期出现。用标记菌株进行融合的半定量分析表明，在HU存在的情况下，融合没有显著的促进作用。在没有HU的情况下，将融合菌落从杂交组合转移到V8++HU中，发现在HU存在的情况下，菌丝生长增强。对核糖核苷酸还原酶HU靶基因表达的分析表明，一个在系统发育上存在分歧的催化亚基是复制应激反应公司。这些结果表明，复制应激的诱导促进了融合后的菌丝生长。H99背景中的New formansvar.grubiistrains。
Mating and sexual development inC. neoformansvar.grubiistrains of the H99 background is often less robust than that laboratory generated isogenicC. neoformansvar.neoformans strains in the JEC21 background. InCandida albicansandSaccharomyces serevisiae, slowing of DNA synthesis and engagement of the replication stress response, such as that caused by treatment with hydroxyurea (HU), induces filamentation and pseudohyphal growth, respectively. In this study, we investigated the effect of HU treatment onC. neoformansvar.grubiimorphogenesis. Treatment with HU did not induce filamentation of yeast cells either in liquid culture or on solid YPD or V8 agar. In the presence of the opposite mating partner, we observed early emergence of hyphae in the presence of HU. Semi-quantitative analysis of fusion using marked strains demonstrated that no significant enhancement of fusion in the presence of HU. Transfer of fusion colonies from crosses performed in the absence of HU to V8 + HU revealed enhanced hyphal growth in the presence of HU. Analysis of expression of the target of HU, ribonucleotide reductase, revealed that a phylogenetically divergent catalytic subunit is replication stress responsive inC. neoformans.These results suggest that induction of replication stress promotes post-fusion hyphal growth ofC. neoformansvar.grubiistrains in the H99 background.