yadBC of Yersinia pestis, a new virulence determinant for bubonic plague
yadBC of Yersinia pestis, a new virulence determinant for bubonic plague
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DOI：
10.1128/iai.00219-07
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发表时间：
2008-02-01
影响因子：
3.1
通讯作者：
Straley, Susan C.
中科院分区：
文献类型：
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作者：
Forman, Stanislav;Wulff, Christine R.;Straley, Susan C.
在所有检测的鼠疫耶尔森氏菌菌株中，粘附素/侵袭素yadA基因均为假基因，而在鼠疫耶尔森氏菌中，粘附素/侵袭素yadA基因均为假基因。鼠疫对上皮细胞具有侵袭性。为了鉴定与YadA在结构和功能上相似的潜在表面蛋白，我们搜索了Y。鼠疫菌基因组开放阅读框与yadA同源，发现3个：双顺反子操纵子yadBC（YPO 1387和YPO 1388）;鼠疫菌CO 92;鼠疫KIM 5），其编码两种推定的表面蛋白，以及YPO 0902，其缺乏信号序列并且可能是无功能的。在这项研究中，我们描述了yadBC调控的特点，并测试了这个操纵子对Y。鼠疫菌的粘附、侵袭和毒力。我们发现，yadBC的损失导致了适度的损失，上皮样细胞的侵袭性和大幅度降低的毒力，淋巴腺鼠疫，但不是肺鼠疫小鼠。
In all Yersinia pestis strains examined, the adhesin/invasin yadA gene is a pseudogene, yet Y. pestis is invasive for epithelial cells. To identify potential surface proteins that are structurally and functionally similar to YadA, we searched the Y. pestis genome for open reading frames with homology to yadA and found three: the bicistronic operon yadBC (YPO1387 and YPO1388 of Y. pestis CO92; y2786 and y2785 of Y. pestis KIM5), which encodes two putative surface proteins, and YPO0902, which lacks a signal sequence and likely is nonfunctional. In this study we characterized yadBC regulation and tested the importance of this operon for Y. pestis adherence, invasion, and virulence. We found that loss of yadBC caused a modest loss of invasiveness for epithelioid cells and a large decrease in virulence for bubonic plague but not for pneumonic plague in mice.