Environmental significance of freshets in reducing Perkinsus marinus infection in eastern oysters Crassostrea virginica:: potential management applications
Environmental significance of freshets in reducing Perkinsus marinus infection in eastern oysters Crassostrea virginica:: potential management applications
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DOI：
10.3354/meps248165
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发表时间：
2003-01-01
影响因子：
2.5
通讯作者：
La Peyre, JF
中科院分区：
文献类型：
--
作者：
La Peyre, MK;Nickens, AD;La Peyre, JF
极端淡水事件对Perkinsus marinus-Crassostrea virginica相互作用的影响尚未得到研究。研究了淡水事件对海洋红球藻感染的影响。因此，在受控实验室实验和实地研究中检查了弗吉尼亚和牡蛎的存活情况。对于实验室实验，牡蛎收集在春季，夏季和冬季从路易斯安那州的R marinus是地方病的地区。牡蛎被放置在2个循环水系统中的盐度和温度类似于他们的收集网站。他们进行了2个盐度处理（新鲜和控制）。通过在48 h内将水的盐度降低至0至1 ppt来模拟新鲜事件，并维持21 d。控制牡蛎保持在初始盐度。在新鲜事件之前对30只牡蛎进行采样，在新鲜事件开始后第7、14和21天对每个处理组（新鲜，对照）30只牡蛎进行采样。牡蛎死亡率，R marinus感染强度，牡蛎条件指数和牡蛎血浆渗透压测定每周样品。所有3个模拟的淡水事件（即春季、夏季、冬季）均导致海泛藻感染强度显著降低，但未能消除感染。牡蛎血浆未能达到极低的渗透压（
The effects of extreme freshwater events on Perkinsus marinus-Crassostrea virginica interactions remain unexplored. The effects of freshwater events on R marinus infection in C. virginica and oyster survival were therefore examined in controlled laboratory experiments and a field study. For the laboratory experiments, oysters were collected in spring, summer and winter from an area in Louisiana where R marinus is endemic. Oysters were placed in 2 recirculating water systems at a salinity and temperature similar to their collection site. They were subjected to 2 salinity treatments (freshet and control). Freshet events were simulated by reducing the water to salinities of 0 to 1 ppt over a 48 h period, and maintained for a 21 d period. Control oysters were maintained at the initial salinity. Thirty oysters were sampled prior to the freshet event, and 30 oysters per treatment group (freshet, control) were sampled on Days 7, 14 and 21 after initiation of the freshet event. Oyster mortality, R marinus infection intensities, oyster condition index and oyster plasma osmolality were measured in weekly samples. All 3 simulated freshet events (i.e. spring, summer, winter) resulted in a significant reduction in P. marinus infection intensity, but failed to eliminate infection. The failure of the oyster plasma to reach very low osmolality (