Controlled Supramolecular Self-Assembly of Super-charged β-Lactoglobulin A-PEG Conjugates into Nanocapsules
Controlled Supramolecular Self-Assembly of Super-charged β-Lactoglobulin A-PEG Conjugates into Nanocapsules
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DOI：
10.1002/anie.201704298
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发表时间：
2017-09-18
影响因子：
16.6
通讯作者：
Nallani, Madhavan
中科院分区：
文献类型：
--
作者：
Khan, Amit Kumar;Gudlur, Sushanth;Nallani, Madhavan
报道了一种由乳球蛋白A(LGA)和聚乙二醇聚乙二醇(PEGg)组成的新型蛋白质-聚合物偶联物的合成和表征。LGA通过胺化或琥珀酸化后与聚乙二醇偶联物的增压作用选择性地修饰为自组装。相反电荷的蛋白质-聚合物的等摩尔混合物自组装成直径80-100 nm的球形胶囊。自组装是通过同时利用蛋白质-聚合物结合物的两亲性和聚电解质性质来进行的。这些蛋白质聚合物胶囊或蛋白质小体类似于蛋白质衣壳，并能够将溶质包裹在其内部。我们预计这种方法也适用于其他球状蛋白。
The synthesis and characterization of a new protein-polymer conjugate composed of lactoglobulinA (LGA) and poly(ethylene glycol) PEG is described. LGA was selectively modified to self-assemble by super-charging via amination or succinylation followed by conjugation with PEG. An equimolar mixture of the oppositely charged protein-polymer conjugates self-assemble into spherical capsules of 80-100nm in diameter. The self-assembly proceeds by taking simultaneous advantage of the amphiphilicity and polyelectrolyte nature of the protein-polymer conjugate. These protein-polymer capsules or proteinosomes are reminiscent of protein capsids, and are capable of encapsulating solutes in their interior. We envisage this approach to be applicable to other globular proteins.