Spirosoma arcticum sp nov., isolated from high Arctic glacial till
Spirosoma arcticum sp nov., isolated from high Arctic glacial till
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Spirosoma arcticum sp nov.，从北极高冰川冰碛中分离出来
DOI：
10.1099/ijs.0.061853-0
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发表时间：
2014-07-01
影响因子：
2.8
通讯作者：
Peng, Fang
中科院分区：
文献类型：
--
作者：
Chang, Xulu;Jiang, Fan;Peng, Fang
从挪威斯瓦尔巴德群岛新奥勒松冰川冰碛中分离到一株橙色菌株，命名为R2-35（T）。细胞需氧，革兰氏阴性，杆状，有时丝状。生长发生在4-28 ° C（最适，20 ° C），pH 7.0-9.0（最适，pH 8.0）和0- 1%NaCl下。基于16 S rRNA基因序列的系统发育分析表明，菌株R2-35（T）属于螺旋体属（Spirosoma），与近缘种的序列相似性为91.65 ~ 95.19%。菌株R2-35（T）含有作为主要细胞脂肪酸的C-16：0（10.7%）、C-18：0（9.2%）、C-16：1 ω 5c（16.5%）和总特征3（C-16：1 ω 6c和/或C-16：1 ω 7 c）（24.6%）、作为主要呼吸醌的MK-7和作为主要极性脂质的磷脂酰乙醇胺。菌株R2-35（T）的DNA G+C含量为54.9mol%。基于系统发育、生理和化学分类学数据，菌株R2-35（T）被认为代表螺旋体属的一个新种，其名称为北极螺旋体（Spirosoma arcticum sp. nov.），模式菌株为R2-35（T）（=CCTCC AB 2012849（T）=LMG 28141（T））。
A novel orange-pigmented strain, designated R2-35(T), was isolated from a glacier till near Ny-Alesund, Svalbard Archipelago, Norway. The cells were aerobic, Gram-negative, rod-shaped and sometimes filamentous. Growth occurred at 4-28 degrees C (optimum, 20 degrees C), at pH 7.0-9.0 (optimum, pH 8.0) and with 0-1 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R2-35(T) belonged to the genus Spirosoma with sequence similarity to related species ranging from 91.65 to 95.19%. Strain R2-35(T) contained C-16:0 (10.7%), C-18:0 (9.2%), C-16:1 omega 5c (16.5 %) and summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) (24.6 %) as the major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain R2-35(T) was 54.9 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R2-35(T) is considered to represent a novel species of the genus Spirosoma, for which the name Spirosoma arcticum sp. nov., is proposed, The type strain is R2-35(T) (=CCTCC AB 2012849(T)=LMG 28141(T)).