Strategy for introduction of foreign genes into field populations of Chagas disease vectors
Strategy for introduction of foreign genes into field populations of Chagas disease vectors
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DOI：
10.1093/aesa/92.6.937
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发表时间：
1999-11-01
影响因子：
2.3
通讯作者：
Cordon-Rosales, C
中科院分区：
文献类型：
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作者：
Durvasula, RV;Kroger, A;Cordon-Rosales, C
副转基因是控制某些节肢动物传播的传染病的一种策略，涉及通过遗传改变昆虫载体的共生细菌表达疾病传播阻断分子。这种方法的田间使用依赖于将遗传转化的细菌传播到其宿主昆虫的方法。Rhodococcus rhodnii是南美锥虫病载体Rhodnius prolixus（Stal）的共生细菌，已被遗传转化以表达几种分子。在这里，我们报告了一个战略的传播转基因R。rhodnii的目标种群的R. prolixus，使用称为CRUZIGARD的合成底物。该制剂模拟了R. prolixus，当浸渍有基因改变的共生细菌时，模拟reduviid错误使用的共生体散布的自然食粪方法。每月将载有遗传改变的共生体的CRUZIGARD应用于封闭的R，prolixus patient笼。在实验室条件下和模拟的现场条件下，其中竞争的环境微生物存在的重组细菌的摄取和保留证明。在危地马拉进行的模拟田间研究中，近50%的R。暴露于载有细菌的CRUZIGARD的prolixus在整个发育过程中保留了转基因微生物。在这些副转基因昆虫中，基因改变的共生体构成了近95%的细菌菌落形成单位。未观察到CRUZIGARD对昆虫发育和繁殖力的不良影响。CRUZIGARD代表了一种潜在的强有力的方法，用于在恰加斯病载体R.冗长
Paratransgenesis, a strategy for control of certain arthropod-borne infectious diseases, involves expression of disease transmission-blocking molecules by genetically altered symbiotic bacteria of insect vectors. Field use of this approach relies on a method for dispersal of genetically transformed bacteria to their host insects. Rhodococcus rhodnii, a symbiotic bacterium of the Chagas disease vector, Rhodnius prolixus (Stal), has been transformed genetically to express several molecules. Here we report a strategy for dispersal of genetically altered R. rhodnii to target populations of R. prolixus that uses a synthetic substrate termed CRUZIGARD. This preparation mimics natural feces of R. prolixus and, when impregnated with genetically altered symbiotic bacteria, simulates the natural coprophagic method of symbiont dispersal used by the reduviid bug. CRUZIGARD laden with genetically altered symbionts was applied monthly to closed cages of R, prolixus nymphs. Uptake and retention of the recombinant bacteria was demonstrated under laboratory conditions and simulated field conditions in which competing environmental microbes were present. In the simulated field study conducted in Guatemala, nearly 50% of R. prolixus nymphs exposed to bacteria-laden CRUZIGARD retained the transgenic microbes throughout their development. In these paratransgenic insects, the genetically altered symbionts comprised nearly 95% of the bacterial colony forming units. No adverse effects of CRUZIGARD on insect development and fecundity were noted. CRUZIGARD represents a potentially powerful method for dispersal of recombinant symbiotic bacteria among field populations of the Chagas disease vector, R. prolixus.