Profiling CD antigens on leukaemias with an antibody microarray
Profiling CD antigens on leukaemias with an antibody microarray
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DOI：
10.1016/j.febslet.2009.03.018
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发表时间：
2009-06-05
期刊：
影响因子：
3.5
通讯作者：
Christopherson, Richard I.
中科院分区：
文献类型：
--
作者：
Barber, Nicole;Gez, Swetlana;Christopherson, Richard I.
当在一组标准人类细胞的一些成员上发现的表面分子与至少一种新抗体反应时，定义分化簇（CD）抗原，并且存在良好的伴随分子数据。白细胞表面CD抗原的单克隆抗体已用于流式细胞术，最近用于构建捕获活细胞的微阵列。这些DotScan（TM）微阵列能够快速和高度平行地表征CD抗原的库，其表达模式可能与离散的白血病亚型相关，或用于定义非血液学疾病的生物标志物“签名”。具有荧光多路复用功能的DotScan（TM）能够分析CD抗原的次要细胞亚群，例如来自手术样本的结肠直肠癌细胞和肿瘤浸润淋巴细胞。(C)2009年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Cluster of differentiation ( CD) antigens are defined when a surface molecule found on some members of a standard panel of human cells reacts with at least one novel antibody, and there is good accompanying molecular data. Monoclonal antibodies to surface CD antigens on leukocytes have been used for flow cytometry, and more recently to construct microarrays that capture live cells. These DotScan (TM) microarrays enable the rapid and highly parallel characterization of repertoires of CD antigens whose expression patterns may be correlated with discrete leukaemia subtypes, or used to define biomarker 'signatures' for non-hematological diseases. DotScan (TM) with fluorescence multiplexing enables profiling of CD antigens for minor subsets of cells, such as colorectal cancer cells and tumour-infiltrating lymphocytes from a surgical sample. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.