Loss of Lhx1 activity impacts on the localization of primordial germ cells in the mouse
Loss of Lhx1 activity impacts on the localization of primordial germ cells in the mouse
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DOI：
10.1002/dvdy.22417
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发表时间：
2010-11
影响因子：
2.5
通讯作者：
Satomi S. Tanaka;Yasuka L. Yamaguchi;K. Steiner;T. Nakano;R. Nishinakamura;K. Kwan;R. Behringer;P. Tam
中科院分区：
文献类型：
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作者：
Satomi S. Tanaka;Yasuka L. Yamaguchi;K. Steiner;T. Nakano;R. Nishinakamura;K. Kwan;R. Behringer;P. Tam
缺乏Lhx1 （Lim1）活性的小鼠胚胎显示原肠胚形成缺陷和原始生殖细胞（PGCs）缺陷（Tsang et al.[2001]国际发育生物学杂志45:549-555）。为了剖析Lhx1在生殖细胞发育中的具体作用，我们研究了外胚层衍生物中Lhx1活性条件失活的胚胎，与完全无效的胚胎相比，这些胚胎通过原肠胚形成正常发育，然后表现出头部截断表型。在条件突变胚胎中，PGCs最初定位于后肠的最终内胚层，但它们过早地离开胚胎肠。PGCs从肠道早期退出伴随着Ifitm1在包围肠道的中胚层中无法维持强表达，这可能介导了排斥活动，促进了PGCs在早期器官发生时在后肠中保留。因此，Lhx1可能通过调节Ifitm1介导的排斥活性来影响PGCs的定位。科学通报，2010,(3):1 - 4。©2010 Wiley‐Liss, Inc。
Mouse embryos lacking Lhx1 (Lim1) activity display defective gastrulation and are deficient of primordial germ cells (PGCs) (Tsang et al. [ 2001 ] International Journal of Developmental Biology 45:549–555). To dissect the specific role of Lhx1 in germ cell development, we studied embryos with conditional inactivation of Lhx1 activity in epiblast derivatives, which, in contrast to completely null embryos, develop normally through gastrulation before manifesting a head truncation phenotype. Initially, PGCs are localized properly to the definitive endoderm of the posterior gut in the conditional mutant embryos, but they depart from the embryonic gut prematurely. The early exit of PGCs from the gut is accompanied by the failure to maintain a strong expression of Ifitm1 in the mesoderm enveloping the gut, which may mediate the repulsive activity that facilitates the retention of PGCs in the hindgut during early organogenesis. Lhx1 therefore may influence the localization of PGCs by modulating Ifitm1‐mediated repulsive activity. Developmental Dynamics 239:2851–2859, 2010. © 2010 Wiley‐Liss, Inc.