Molecular phylogeny and PSP toxin profile of the Alexandrium tamarense species complex along the coast of China.
Molecular phylogeny and PSP toxin profile of the Alexandrium tamarense species complex along the coast of China.
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DOI：
10.1016/j.marpolbul.2014.09.056
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发表时间：
2014-12
影响因子：
5.8
通讯作者：
Cheng Zou;R. Ye;Jian-wei Zheng;Zhaohui Luo;H. Gu;Wei-dong Yang;Hong-ye Li;Jie‐sheng Liu
中科院分区：
文献类型：
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作者：
Cheng Zou;R. Ye;Jian-wei Zheng;Zhaohui Luo;H. Gu;Wei-dong Yang;Hong-ye Li;Jie‐sheng Liu
为了解我国沿海塔玛亚历山大藻（Alexaculum tamarens species complex）沿着的遗传多样性和麻痹性贝毒（PSP）毒素谱，对67株塔玛亚历山大藻（Alexaculum tamarens species complex）进行了遗传多样性分析。从中国近海采集塔玛藻（tamarens），利用rDNA序列分析了塔玛藻的遗传多样性。此外，采用高效液相色谱法测定了PSP毒素的组成和含量。根据5.8S rDNA、ITS和LSU rDNA D1-D2序列，A. tamarensin中国海至少包括组IV和组I核糖体型。在这些中国菌株中，毒素谱中浓度最高的是C1/2、gonyautoxins 1/4（GTX 1/4）和新石房蛤毒素（NEO）。但毒素谱不典型，部分菌株未检出C1/2毒素。PSP毒素谱与地理分布之间没有严格的相关性。
To explore the genetic diversity and paralytic shellfish poisoning (PSP) toxin profile of theAlexandrium tamarensespecies complex along the coast of China, 67 strains ofA. tamarensefrom the China Sea were collected and genetic diversity were analyzed based on the rDNA sequences. In addition, PSP toxin compositions and contents were detected by HPLC. According to the 5.8S rDNA and ITS, and LSU rDNA D1–D2 sequence,A. tamarensein the China Sea comprises at least Group IV and Group I ribotypes. In these Chinese strains, the toxins with the highest concentration in the profile were C1/2, gonyautoxins 1/4 (GTX1/4) and neosaxitoxin (NEO). However, the toxin profiles were atypical and C1/2 toxins were not detected in some strains. No strict correlation was observed between the PSP toxins profile and the geographical distribution.