The first completed genome sequence from a teleost fish (Fugu rubripes) adds significant diversity to the nuclear receptor superfamily
The first completed genome sequence from a teleost fish (Fugu rubripes) adds significant diversity to the nuclear receptor superfamily
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DOI：
10.1093/nar/gkg444
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发表时间：
2003-07-15
影响因子：
14.9
通讯作者：
Ramamurthy, L
中科院分区：
文献类型：
--
作者：
Maglich, JM;Caravella, JA;Ramamurthy, L
定义来自不同物种的基因家族成员的完整集合为比较研究提供了基础。使用生物信息学的方法，我们已经定义了整个核受体补体内的第一个可用的完整序列的非人类脊椎动物（硬骨鱼红鳍东方鲀）。与人类组（48个总核受体）相比，我们在河豚基因组中发现了68个核受体。所有68个河豚受体都有明确的人类同源物，因此没有定义新的核受体亚群。倒数分析显示，除了CAR（NR 1 I3）和LXR β（NR 1H 2）之外，每个人类受体都有一个或多个河豚直系同源物。这68种受体为已知的核受体超家族增加了惊人的多样性，并为人类核受体提供了重要的比较物。我们已经比较了几种与人类核受体相关的人核受体（FXR、LXR α/β、CAR、PXR、VDR和PPAR α/γ/δ）与它们的河豚直系同源物。该比较包括五种河豚组织类型的表达分析。在本研究中通过PCR分析的所有河豚鱼受体均表达，表明大多数其他河豚鱼受体可能是功能性的。
Defining complete sets of gene family members from diverse species provides the foundation for comparative studies. Using a bioinformatic approach, we have defined the entire nuclear receptor complement within the first available complete sequence of a non-human vertebrate (the teleost fish Fugu rubripes). In contrast to the human set (48 total nuclear receptors), we found 68 nuclear receptors in the Fugu genome. All 68 Fugu receptors had a clear human homolog, thus defining no new nuclear receptor subgroups. A reciprocal analysis showed that each human receptor had one or more Fugu orthologs, excepting CAR (NR1I3) and LXRbeta (NR1H2). These 68 receptors add striking diversity to the known nuclear receptor superfamily and provide important comparators to human nuclear receptors. We have compared several pharmacologically relevant human nuclear receptors (FXR, LXRalpha/beta, CAR, PXR, VDR and PPARalpha/gamma/delta) to their Fugu orthologs. This comparison included expression analysis across five Fugu tissue types. All of the Fugu receptors that were analyzed by PCR in this study were expressed, indicating that the majority of the additional Fugu receptors are likely to be functional.