700-nm Zwitterionic Near-Infrared Fluorophores for Dual-Channel Image-Guided Surgery.
700-nm Zwitterionic Near-Infrared Fluorophores for Dual-Channel Image-Guided Surgery.
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DOI：
10.1007/s11307-015-0870-4
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发表时间：
2016-02
影响因子：
3.1
通讯作者：
Choi HS
中科院分区：
文献类型：
--
作者：
Hyun H;Henary M;Gao T;Narayana L;Owens EA;Lee JH;Park G;Wada H;Ashitate Y;Frangioni JV;Choi HS
开发一系列700 nm两性离子五甲川吲哚菁近红外荧光团，以实现双通道图像引导手术。三个互补的合成方案被用来产生新的两性离子化学结构。物理化学，光学，生物分布和清除性能进行了比较，Cy5.5，一个传统的五甲川吲哚菁现在用于生物医学成像。ZW 700 -1a、ZW 700 -1b和ZW 700 -1c被合成、纯化并在体外和体内进行了广泛的分析。所有分子的消光系数≥ 199，000 M−1cm−1，发射≥ 660 nm，在温血清中24 h后稳定性≥ 99%。在小鼠、大鼠和猪中，≥ 80%的注射剂量在4 h内通过肾脏清除率从体内完全消除。无论是单独使用还是与肿瘤靶向配体结合，ZW 700 -1a都可以使用FLARE®成像系统实现双通道、高SBR和800 nm NIR荧光团同步成像。新型700 nm两性离子NIR荧光团实现双NIR图像引导手术。
To develop a family of 700 nm zwitterionic pentamethine indocyanine near-infrared fluorophores that would permit dual-channel image-guided surgery. Three complementary synthetic schemes were used to produce novel zwitterionic chemical structures. Physicochemical, optical, biodistribution, and clearance properties were compared to Cy5.5, a conventional pentamethine indocyanine now used for biomedical imaging. ZW700-1a, ZW700-1b, and ZW700-1c were synthesized, purified, and analyzed extensively in vitro and in vivo. All molecules had extinction coefficients ≥ 199,000 M−1cm−1, emission ≥ 660 nm, and stability ≥ 99% after 24 h in warm serum. In mice, rats, and pigs, ≥ 80% of the injected dose was completely eliminated from the body via renal clearance within 4 h. Either alone or conjugated to a tumor targeting ligand, ZW700-1a permitted dual-channel, high SBR, and simultaneous imaging with 800 nm NIR fluorophores using the FLARE® imaging system. Novel 700 nm zwitterionic NIR fluorophores enable dual-NIR image-guided surgery.