Effects of etanercept, a tumour necrosis factor-α antagonist, in an experimental model of periodontitis in rats
Effects of etanercept, a tumour necrosis factor-α antagonist, in an experimental model of periodontitis in rats
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DOI：
10.1038/sj.bjp.0706979
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发表时间：
2007-02-01
影响因子：
7.3
通讯作者：
Cuzzocrea, S.
中科院分区：
文献类型：
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作者：
Di Paola, R.;Mazzon, E.;Cuzzocrea, S.
背景和目的：依那西普是一种具有抗炎作用的肿瘤坏死因子拮抗剂。我们的研究的目的是评估，第一次，在体内抑制TNF-α的实验modelofperiodontis.Experimental方法的治疗效果：牙周炎是诱导成年雄性SD大鼠通过放置尼龙线结扎周围较低的第一磨牙。依那西普的给药剂量为5 mg/kg，s. c.将该混合物干燥，关键结果：大鼠牙周炎导致炎症过程，其特征在于水肿、中性粒细胞浸润和细胞因子产生，随后是其他炎症细胞的募集、一系列炎症介质的产生、组织损伤、细胞凋亡和疾病。依那西普（5 mg/kg，s. c.将该混合物干燥，结扎后）显著降低了（1）牙周炎炎症和组织损伤的程度（组织学评分），（2）中性粒细胞浸润（MPO评价），（3）iNOS（硝基酪氨酸和细胞因子（例如TNF-α）的表达）和（4）细胞凋亡（Bax和Bcl-2表达）。结论和意义：总之，我们的结果清楚地表明，依那西普治疗减少了炎症和组织损伤的发展，与牙周炎相关的事件。
Background and purpose: Etanercept is a tumour necrosis factor antagonist with anti-inflammatory effects. The aim of our study was to evaluate, for the first time, the therapeutic efficacy of in vivo inhibition of TNF-alpha in an experimental model of periodontitis.Experimental approach: Periodontitis was induced in adult male Sprague-Dawley rats by placing a nylon thread ligature around the lower 1st molars. Etanercept was administered at a dose of 5 mg kg(-1), s. c., after placement of the ligature.Key results: Periodontitis in rats resulted in an inflammatory process characterized by oedema, neutrophil infiltration and cytokine production that was followed by the recruitment of other inflammatory cells, production of a range of inflammatory mediators, tissue damage, apoptosis and disease. Treatment of the rats with etanercept (5 mg kg(-1), s. c., after placement of the ligature) significantly reduced the degree of (1) periodontitis inflammation and tissue injury (histological score), (2) infiltration of neutrophils (MPO evaluation), (3) iNOS (the expression of nitrotyrosine and cytokines (eg TNF-alpha)) and (4) apoptosis (Bax and Bcl-2 expression).Conclusions and Implications: Taken together, our results clearly demonstrate that treatment with etanercept reduces the development of inflammation and tissue injury, events associated with periodontitis.