Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation
Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation
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DOI：
10.1016/j.jim.2009.06.001
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发表时间：
2009-08-15
影响因子：
2.2
通讯作者：
Elkord, Eyad
中科院分区：
文献类型：
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作者：
Elkord, Eyad
外周血单个核细胞（PBMC）的冷冻保存对于许多临床和研究测定是必不可少的。一些研究报告了冷冻保存后PBMC表型的一致变化。我们假设PBMC冷冻可能对调节性T细胞（Treg）频率的估计产生负面影响。在从6个健康供体分离的6个新鲜PBMC样品中测量Treg水平，并在冷冻三周后重新测量这些水平。在此，我们报告了冷冻保存后所有样品中Treg频率的显著降低。(C)2009 Elsevier B.V.保留所有权利。
Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation. (C) 2009 Elsevier B.V. All rights reserved.