Activation of the phasic pontine-wave generator enhances improvement of learning performance: a mechanism for sleep-dependent plasticity
Activation of the phasic pontine-wave generator enhances improvement of learning performance: a mechanism for sleep-dependent plasticity
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DOI：
10.1046/j.1460-9568.2003.02460.x
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发表时间：
2003-01-01
影响因子：
3.4
通讯作者：
Datta, S
中科院分区：
文献类型：
--
作者：
Mavanji, V;Datta, S
本研究的目的是检验以下假设：快速眼动 (REM) 睡眠期间补充激活相性脑桥波 (P 波) 发生器可增强记忆的巩固和整合，从而提高学习能力。为了检验这一假设，两组老鼠在早上接受了双向主动回避学习任务的训练。训练结束后，一组大鼠立即在 P 波发生器中显微注射卡巴胆碱，另一组则在同一目标区域显微注射对照盐水。经过训练试验和显微注射后，大鼠被允许在测谎记录室中不受干扰地睡眠 6 小时。在 6 小时不受干扰的睡眠-觉醒记录结束后，老鼠在回避学习试验中接受了重新测试。经过学习试验后，显着注射了盐水（15.36％）和卡巴胆碱（17.70％）的大鼠的快速眼动睡眠时间总百分比显着增加。经过学习试验后，在整个 6 小时的记录期间，用卡巴胆碱治疗的大鼠的 REM 睡眠 P 波密度明显高于用盐水治疗的大鼠。在重试过程中，显微注射卡巴胆碱的大鼠的学习任务表现比显微注射生理盐水的大鼠高 22.75%。这些发现表明，记忆的巩固和整合产生了对 P 波的稳态需求。此外，这些发现首次提供了实验证据，证明 P 波发生器的激活可以增强记忆的巩固和整合，从而提高最近学习的任务的表现。
The aim of this study was to test the hypothesis that supplementary activation of the phasic pontine wave (P-wave) generator during rapid eye movement (REM) sleep enhances consolidation and integration of memories, resulting in improved learning. To test this hypothesis, two groups of rats were trained on a two-way active avoidance learning task in the morning. Immediately after training, one group of rats received a carbachol microinjection into the P-wave generator and the other group was microinjected with control saline into the same target area. After training trials and microinjections, rats were allowed a 6-h period of undisturbed sleep in the polygraphic recording chamber. At the end of 6 h of undisturbed sleep-wake recordings, rats were retested in a session of avoidance learning trials. After learning trials, the total percentage of time spent in REM sleep was significantly increased in both saline (15.36%)- and carbachol (17.70%)-microinjected rats. After learning trials, REM sleep P-wave density was significantly greater throughout the 6-h period of recordings in carbachol treated rats than in the saline treated rats. In the retrial session, the improvement in learning task performance was 22.75% higher in the carbachol-microinjected rats than in the saline-microinjected rats. These findings show that the consolidation and integration of memories create a homeostatic demand for P-waves. In addition, these findings provide experimental evidence, for the first time, that activation of the P-wave generator may enhance consolidation and integration of memories, resulting in improved performance on a recently learned task.