Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules.
Glutamine synthetase genes of pea encode distinct polypeptides which are differentially expressed in leaves, roots and nodules.
复制标题
DOI：
10.1002/j.1460-2075.1987.tb04710.x
复制
发表时间：
1987-01
期刊：
影响因子：
--
通讯作者：
S. Tingey;E. Walker;G. Coruzzi
中科院分区：
文献类型：
--
作者：
S. Tingey;E. Walker;G. Coruzzi
我们对豌豆各个器官中编码谷氨酰胺合成酶 (GS) 的独特多肽、初级翻译产物和 mRNA 进行了表征。可溶性蛋白的蛋白质印迹分析已鉴定出五种不同的GS多肽，它们在豌豆的叶、根和根瘤中以不同的相对水平表达。在叶子中的两种 GS 多肽（44 和 38 kd）中，44-kd GS 多肽占主导地位，并且定位于叶绿体基质。在根中，主要的 GS 多肽为 38 kd。根瘤菌感染根后，相对于未感染的根，根瘤中三种 37-kd GS 多肽的丰度增加。编码三种不同 GS mRNA 的 cDNA 克隆已得到表征。混合选择翻译已鉴定出三种不同的 GS 初级翻译产物（49、38 和 37 kd）。两个 cDNA 克隆（pGS134 和 pGS341）与根节中最丰富的 GS mRNA 同源，编码 38-和 37-kd GS 初级翻译产物。第三个 cDNA (pGS197) 对应于叶 Poly(A) RNA 特异的较大 GS mRNA 种类，它编码成熟叶绿体 GS 多肽的 49-kd 推定前体。豌豆核DNA的cDNA序列分析和Southern印迹分析鉴定出豌豆中至少三个编码GS的基因，它们相关但在结构和体内表达模式上不同。
We have characterized the distinct polypeptides, primary translation products and mRNAs encoding glutamine synthetase (GS) in the various organs of pea. Western blot analysis of soluble protein has identified five distinct GS polypeptides which are expressed at different relative levels in leaves, roots and nodules of pea. Of the two GS polypeptides in leaves (44 and 38 kd), the 44‐kd GS polypeptide is predominant and is localized to the chloroplast stroma. In roots, the predominant GS polypeptide is 38 kd. Upon Rhizobium infection of roots, three 37‐kd GS polypeptides increase in abundance in the nodules relative to uninfected roots. cDNA clones encoding three different GS mRNAs have been characterized. Hybrid‐select translation has identified three different GS primary translation products (49, 38 and 37 kd). Two cDNA clones (pGS134 and pGS341) are homologous to GS mRNAs most abundant in nodules which encode the 38‐ and 37‐kd GS primary translation products. A third cDNA (pGS197) corresponds to a larger GS mRNA species specific to leaf poly(A) RNA, which encodes a 49‐kd putative precursor to the mature chloroplast GS polypeptide. cDNA sequence analysis and Southern blot analysis of pea nuclear DNA identifies at least three genes encoding GS in pea which are related but distinct in structure and in vivo pattern of expression.