Extracellular Vesicles Carry HIV Env and Facilitate Hiv Infection of Human Lymphoid Tissue.
Extracellular Vesicles Carry HIV Env and Facilitate Hiv Infection of Human Lymphoid Tissue.
复制标题
DOI：
10.1038/s41598-017-01739-8
复制
发表时间：
2017-05-10
影响因子：
4.6
通讯作者：
Margolis L
中科院分区：
文献类型：
--
作者：
Arakelyan A;Fitzgerald W;Zicari S;Vanpouille C;Margolis L
被HIV-1有效感染的细胞释放病毒粒子沿着细胞外囊泡（EV），其生物起源、大小和物理性质类似于逆转录病毒。在这里，我们发现HIV-1感染细胞释放的大量EV（外泌体）携带gp 120（Env），这是一种介导病毒附着和融合到靶细胞的病毒蛋白，也以各种间接方式促进HIV感染。EV的病毒制剂的消耗，特别是携带gp 120的那些，减少了离体人淋巴组织的病毒感染。因此，在我们的工作中发现的携带Env的EV似乎有助于HIV感染，因此可能构成抗病毒策略的新治疗靶点。
Cells productively infected with HIV-1 release virions along with extracellular vesicles (EVs) whose biogenesis, size, and physical properties resemble those of retroviruses. Here, we found that a significant number of EVs (exosomes) released by HIV-1 infected cells carry gp120 (Env), a viral protein that mediates virus attachment and fusion to target cells, and also facilitates HIV infection in various indirect ways. Depletion of viral preparations of EVs, in particular of those that carry gp120, decreases viral infection of human lymphoid tissue ex vivo. Thus, EVs that carry Env identified in our work seem to facilitate HIV infection and therefore may constitute a new therapeutic target for antiviral strategy.