Ultrastructural observations of spermatogenesis in mice resulting from transplantation of mouse spermatogonia.
Ultrastructural observations of spermatogenesis in mice resulting from transplantation of mouse spermatogonia.
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DOI：
10.1002/j.1939-4640.1996.tb01844.x
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发表时间：
1996-11
影响因子：
--
通讯作者：
Lonnie D. Russell;L. R. França;R. Brinster
中科院分区：
文献类型：
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作者：
Lonnie D. Russell;L. R. França;R. Brinster
本研究的目的是提供生殖细胞移植到另一只小鼠的生精管腔后精子发生的形态学特征。受体小鼠（W位点）是不育的，因为在胚胎发育过程中几乎所有生殖细胞前体都未能迁移到生殖嵴，导致精子发生缺陷。管内注射了来自 C57 小鼠的睾丸细胞悬浮液的受体小鼠被允许发育超过 1 年，随后处死动物并通过光学和电子显微镜检查睾丸组织。从横截面的小管中观察，供体小鼠细胞形成正常的细胞联合（阶段）。精原细胞仅在基底室中发现，表明它们从肾小管腔通过支持细胞连接处转移，最终驻留在基底层上。从定量和定性的角度来看，一些肾小管看起来完全正常。其他人则表现出质量和数量上的损害。在一些小管中，细胞联合中缺少一代细胞。多种退化细胞和结构异常是造成这种损伤的原因，然而，最常见的异常发生在精子发生的伸长阶段。这些细胞的伸长异常和随后的退化导致伸长精子细胞的数量少于预期。睾丸的某些区域没有观察到精子发生，仅存在支持细胞。这些区域通常是在未暴露于注射的生殖细胞的动物中观察到的睾丸组织学的典型区域。然而，这些区域的支持细胞吞噬小管生精活跃区域产生的精子。由于来自新鲜或冷冻细胞的生殖细胞移植在生物学和医学中具有广泛的影响，因此精原细胞移植的表征是改进该过程的重要一步。
The objective of the present study was to provide a morphological characterization of spermatogenesis following germ cell transplantation into the seminiferous tubular lumen of another mouse. The recipient mice (W-locus) were sterile because of a defect in spermatogenesis resulting from the failure of virtually all germ cell precursors to migrate to the genital ridge during embryonic development. Recipient mice containing intratubular injections of testis cell suspensions from C57 mice were allowed to develop for over 1 year, whereupon animals were sacrificed and testis tissue examined by light and electron microscopy. Donor mouse cells formed normal cell associations (stages) as viewed in cross-sectioned tubules. Spermatogonia were found exclusively in the basal compartment, indicating that they were translocated from the tubule lumen through the Sertoli cell junctions, eventually to reside on the basal lamina. Some tubules looked entirely normal from both a quantitative and qualitative standpoint. Others showed qualitative and quantitative impairment. In some tubules a generation of cells was missing from a cell association. A variety of degenerating cells and structural abnormalities were responsible for this impairment, however, the most common abnormalities were seen during the elongation phase of spermatogenesis. Elongation abnormalities and the subsequent degeneration of these cells led to the presence of fewer-than-expected elongate spermatids. There were regions of the testis where no spermatogenesis was noted and only Sertoli cells were present. These regions were generally typical of the testis histology seen in animals not exposed to injected germ cells. However, Sertoli cells in these regions phagocytosed sperm produced in spermatogenically active regions of the tubules. Because transplantation of germ cells, either from fresh or from frozen cells, had wide-ranging implications in biology and medicine, characterization of spermatogonial transplants is an important step in improving this procedure.