The fate of the filaria litomosoides sigmodontis in susceptible and naturally resistant mice
The fate of the filaria litomosoides sigmodontis in susceptible and naturally resistant mice
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DOI：
10.1051/parasite/1996031025
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发表时间：
1996-03-01
期刊：
影响因子：
2.9
通讯作者：
Bain, O
中科院分区：
文献类型：
--
作者：
Marechal, P;LeGoff, L;Bain, O
在易感BALB/c和耐药B10 D2小鼠中比较了Litomosoides sigmodontis的命运，呈现相同的主要组织相容性复合体（H-2（d）），试图分离生命周期的不同元素，以便稍后分离所涉及的不同机制。每只雌性小鼠接种一次小剂量感染性幼虫（25 L3）或大剂量[100或200 L3]。总共研究了92只BALB/c和49只B1 OD 2。D10 D2和BALB/c小鼠的早期结局相似;特别是在感染后第一个月内，蠕虫的恢复率几乎相同。占接种幼虫的四分之一。在B10 D2小鼠的阻力出现渐进性，作为判断的第四蜕皮，非常小的不育雌性蠕虫和雄性蠕虫异常左骨针的存在下，和高频率的活丝虫包被炎性细胞和封装死蠕虫的生长迟缓酸。洛杉矶B10 D2中乙状结肠的寿命约为BALB/c中乙状结肠的一半。在感染100-200个L3后，进行尸检直至D20。在BALB/c小鼠中，恢复率增加。在B10 D2小鼠中，生长较早时被阻滞，这种拥挤效应在D10时已经明显;这可能表明代谢因素的作用。两种小鼠品系的生命周期模式证实了最近关于盘尾丝虫的结论：在“大规模破坏的第1阶段”的第二天就确定了恢复率，然后在“死亡率不显著的第2阶段”保持稳定。在第1阶段，如果感染性幼虫不能通过穿透局部淋巴管而逃离炎症过程，则它们立即在皮下组织中被破坏。相比之下，第二阶段，这是比第三幼虫阶段的持续时间长，表明没有死亡率与第三蜕皮，至少在一次接种。
The fate of Litomosoides sigmodontis was compared in susceptible BALB/c and resistant B10D2 mice, presenting the same major histocompatibility complex (H-2(d)), with an attempt to dissociate the different elements of the life cycle in order, later, to dissociate the different mechanisms involved. Each female mouse was inoculated once with a small dose of infective larvae (25 L3) or a large dose [100 or 200 L3). In total, 92 BALB/c and 49 B1OD2 were studied.Necropsies were performed up to D85 following infection with 25 larvae. The early fate was similar in D10D2 and BALB/c mice; particularly the recovery rate of worms was almost identical during the first month p.i. and represented a quarter of the inoculated larvae. Resistance in B10D2 mice appeared progressively, as judged by retardation of growth acid of the fourth moulting, the presence of very small sterile female worms and male worms with abnormal left spicule, and a high frequency of live filariae coated with inflammatory cells and encapsulated dead worms. The L. sigmodontis life span in B10D2 was about half that in BALB/c.Necropsies were carried out up to D20 following infection with 100-200 L3. The recovery rate was increased in BALB/c. Growth was retarded earlier in B10D2 mice, this crowding effect already apparent at D10; this may indicate a role for metabolic factors. The pattern of the life cycle in both mouse strains confirms recent conclusions on Onchocercinae: the recovery rate is established as soon as the second day during ''phase 1 of massive destruction'', then ii is stable during ''phase 2 of insignificant mortality''. During phase 1, the infective larvae are immediately destroyed in the subcutaeous tissue if they are not able to escape the inflammatory process by penetrating in local lymphatic vessels. By contrast, phase 2, which is longer than the duration of the third larval stage, indicates there is no mortality linked io the third moulting, at least following a single inoculation.