Presence of an unique IgT on the IGH locus in three-spined stickleback fish (Gasterosteus aculeatus) and the very recent generation of a repertoire of VH genes
Presence of an unique IgT on the IGH locus in three-spined stickleback fish (Gasterosteus aculeatus) and the very recent generation of a repertoire of VH genes
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DOI：
10.1016/j.dci.2009.08.011
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发表时间：
2010-02-01
影响因子：
2.9
通讯作者：
Magadan-Mompo, Susana
中科院分区：
文献类型：
--
作者：
Gambon-Deza, Francisco;Sanchez-Espinel, Christian;Magadan-Mompo, Susana
本研究描述了Gasterosteus aculeatus的IGH基因座，10个基因编码三种免疫球蛋白：IgT，IgM和IgD。这些基因被组织成具有由包括VH区段的区段分隔的IGHT-IGHM-IGHD的三个重复的结构。还有第四个基因。IGHT编码具有三个免疫球蛋白结构域的抗体。比较研究表明，它与位于硬骨鱼IGHM上游的IgT和IgZ等抗体有关。IGHM和IGHD与硬骨鱼中描述的相似。IGHM有四个免疫球蛋白结构域，而IGHD有七个，没有一个是重复的。IGH基因座位于G. Aculeatus有49个VH片段，分布在四个区域。它们属于四个家族，其成员显示大于92%的氨基酸同一性，表明VH家族最近多样化。系统发育重建表明它们起源于4个VH片段，这4个VH片段必须与恒定区基因复制，然后这4个VH片段产生其余片段。这表明存在在VH区中产生多样性的活性生物过程。(C)2009爱思唯尔有限公司版权所有。
This study describes the IGH locus in Gasterosteus aculeatus, with 10 genes encoding three immunoglobulin classes: IgT, IgM and IgD. These genes are organized into a structure with three repeats of IGHT-IGHM-IGHD separated by segments including the VH segments. There was also a fourth IGHT gene. IGHT encodes an antibody with three immunoglobulin domains. Comparative studies indicate it is related to IgT and IgZ and other antibodies located upstream of the IGHM in teleost fish. The IGHM and IGHD are similar to the ones described in teleost. The IGHM has four immunoglobulin domains while the IGHD seven and none is duplicated. The IGH locus of G. aculeatus has 49 VH segments located in four regions. They belonged to four families, whose members show a greater than 92% amino acid identity, indicating that VH families diversified recently. Phylogenetic reconstruction suggests they were originated from four VH segments that must have duplicated with the constant region genes, after that the four VH segments gave rise to the remaining segments. This suggests the presence of an active biological process that generates diversity in VH regions. (C) 2009 Elsevier Ltd. All rights reserved.