Clinicopathological significance of miR-101-3p and c-myc mRNA expression in diffused large B-cell lymphoma
Clinicopathological significance of miR-101-3p and c-myc mRNA expression in diffused large B-cell lymphoma
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弥漫性大B细胞淋巴瘤中miR-101-3p和c-myc mRNA表达的临床病理意义
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Xinxia Li
中科院分区：
文献类型：
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作者：
Wenli Cui;Xingyan Zhang;Shutao Zheng;Zhiping Ma;Xuelian Pang;Xinxia Li
MiR-101- 3 p被认为与淋巴瘤的发病机制有关，然而关于其在弥漫性大B细胞淋巴瘤（DLBCL）中的表达的临床病理学意义知之甚少。相反，尽管c-myc在DLBCL中已被广泛研究，但也没有关于临床病理学意义的直接证据。鉴于此，本研究采用实时荧光定量PCR方法检测100例DLBCL组织中miR-101- 3 p和c-myc mRNA水平的表达，以了解miR-101- 3 p和c-myc在DLBCL中的表达意义。使用交叉表检验统计分析miR-101- 3 p和c-myc表达与临床病理变量之间的关联。结果显示，miR-101- 3 p表达仅与组织病理学亚型和治疗方案显著相关，与其他临床病理变量无显著相关性。c-myc表达仅与性别、IPI和血清活性显著相关，与其他临床病理变量无显著相关性。LDH.in总之，我们的研究提供了关于DLBCL中miR-101- 3 p和c-myc表达的临床病理学意义的直接证据，这需要在不同队列中用更大的样本量进一步证实。
MiR-101-3p has been suggested to be implicated in the pathogenesis of lymphoma, however little is.known regarding clinicopathological significance of its expression in diffused large B-cell lymphoma (DLBCL). In.contrast, although c-myc has been extensively studied in DLBCL, no direct evidence concerning clinicopathological.significance has been well established, either. Given this, in our present study, to understand the significance.of both miR-101-3p and c-myc expression on mRNA level in DLBCL, real-time quantitative PCR was used to detect.the expression of miR-101-3p and c-myc on mRNA level in 100 cases of DLBCL samples. Association between.expression of miR-101-3p and c-myc and clinicopathological variables available was statistically analyzed using.Cross-Table test. It was shown that only significant association was observed between miR-101-3p expression and.histopathological subtype and therapeutic regimen, no significant relationship was found with other clinicopathological.variables. As for c-myc expression, only significant association was found with gender, IPI, and activity of LDH.in serum; No significant relations were found with other clinicopathological variables. Together, our study presents.the direct evidence regarding clinicopathological significance of miR-101-3p and c-myc expression in DLBCL, which.warrants further confirmation in different cohorts with larger sample sizes.