Peroxisome proliferator-activated receptor activators modulate the osteoblastic maturation of MC3T3-E1 preosteoblasts
Peroxisome proliferator-activated receptor activators modulate the osteoblastic maturation of MC3T3-E1 preosteoblasts
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DOI：
10.1016/s0014-5793(00)01372-7
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发表时间：
2000-04-07
期刊：
影响因子：
3.5
通讯作者：
Demer, LL
中科院分区：
文献类型：
--
作者：
Jackson, SM;Demer, LL
在骨质疏松症中观察到的骨矿物质密度（BMD）的降低部分是由于成骨细胞活性的降低。我们检测了过氧化物酶体增殖物激活受体（PPAR）激活剂对MC 3 T3-E1前成骨细胞成熟的影响。通过逆转录-聚合酶链反应测定，PPAR α、δ和γ的激活剂诱导碱性磷酸酶活性、基质钙化和成骨细胞基因表达。Howe，呃，在相对高浓度的特定PPAR γ配体环格列酮和曲格列酮下，成熟受到抑制。在MC 3 T3-E1细胞中表达PPARalpha、delta和gamma 1。在成骨细胞成熟过程中，过氧化物酶体增殖物激活物受体γ 1的mRNA和蛋白水平在早期就被诱导。我们推测内源性和药理学的过氧化物酶体增殖物激活物可能通过调节成骨细胞的成熟而影响骨密度。(C)2000年欧洲生物化学学会联合会。
The reduced bone mineral density (BMD) observed in osteoporosis results, in part, from reduced activity of bone-forming osteoblasts, We examined the effect of peroxisome proliferator-activated receptor (PPAR) activators on MC3T3-E1 preosteoblast maturation. Activators of PPAR alpha, delta and gamma induced alkaline phosphatase activity, matrix calcification and the expression of osteoblast genes as determined by reverse transcriptase-polymerase chain reaction, Howe, er, at relatively high concentrations of the specific PPAR gamma ligands, ciglitazone and troglitazone, maturation was inhibited. PPAR alpha, delta and gamma 1 were expressed in MC3T3-E1 cells. PPAR gamma 1 mRNA and protein levels were induced early during osteoblastic maturation, We speculate that endogenous and pharmacological PPAR activators mag affect BMD by modulating osteoblastic maturation. (C) 2000 Federation of European Biochemical Societies.