Resonant four-wave mixing of gold nanoparticles for three-dimensional cell microscopy
Resonant four-wave mixing of gold nanoparticles for three-dimensional cell microscopy
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DOI：
10.1364/ol.34.001816
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发表时间：
2009-06-15
期刊：
影响因子：
3.6
通讯作者：
Borri, Paola
中科院分区：
文献类型：
--
作者：
Masia, Francesco;Langbein, Wolfgang;Borri, Paola
通过检测金纳米粒子与其表面等离子体共振产生的瞬态四波混频，我们证明了一种适用于细胞显微镜的多光子成像模式。四波混频测量自由使用三光束激发几何和外差检测的背景。我们实现了140 mn的面内和470 nm的轴向方向的空间分辨率，超过了单光子衍射极限。用这种技术，高对比度的高尔基体结构的光稳定成像证明在HepG2细胞标记的金纳米粒子的10 nm和5 nm的直径。(C)2009年美国光学学会
By detecting the transient four-wave mixing from gold nanoparticles in resonance with their surface plasmon, we demonstrate a multiphoton imaging modality suited for cell microscopy. Four-wave mixing is measured free from background using a three-beam excitation geometry and heterodyne detection. We achieve a spatial resolution of 140 mn in-plane and 470 nm in the axial direction, surpassing the one-photon diffraction limit. With this technique, high-contrast photostable imaging of Golgi structures is demonstrated in HepG2 cells labeled with gold nanoparticles of 10 mn and 5 nm diameter. (C) 2009 Optical Society of America