Characterization of the antigen recognized by a monoclonal antibody MN9: unique transport pathway to the equatorial segment of sperm head during spermiogenesis
Characterization of the antigen recognized by a monoclonal antibody MN9: unique transport pathway to the equatorial segment of sperm head during spermiogenesis
复制标题
单克隆抗体 MN9 识别的抗原的表征：精子发生过程中精子头部赤道段的独特运输途径
作者：
Kiyotaka Toshimori;I. Tanii;Shukuro Araki;C. Ōura
ymn9是一种针对小鼠精子的单克隆抗体，可特异性识别包括人类在内的几种哺乳动物精子头赤道段。小鼠精子胶体金免疫电镜显示抗原定位于顶体内外膜间隙和顶体膜赤道段。附睾尾精子电泳后免疫印迹鉴定出小鼠和大鼠分别为38 kDa和48 kDa两条免疫反应带。在大鼠精子发生过程中，该抗原通过独特的途径转运到赤道段，首先出现在内质网的一些池中，并在第3步左右出现在精子的高尔基体中。抗原还可在高尔基体外侧的囊泡、头帽基质和第4步至第7步精细胞的头帽膜上发现。在精子发生后期，抗原似乎集中在赤道段。在这一途径中既不需要（前）顶体颗粒也不需要周围的膜。这一通路被称为“高尔基-头帽束”。
SummaryMN9, a monoclonal antibody raised against mouse spermatozoa, specifically recognizes the equatorial segment of sperm head in several mammalian species, including humans. Colloidal gold-immuno-electron microscopy of mouse spermatozoa has shown that the antigen is localized in the space between the outer and inner acrosome membranes and on the acrosome membranes at the equatorial segment. Immunoblotting after electrophoresis of spermatozoa from the cauda epididymidis has identified two immunoreactive bands: 38 kDa and 48 kDa in mouse, and 48 kDa in rat. During spermiogenesis in rat, this antigen is transported to the equatorial segment via a unique pathway, first appearing in some cisternae of the endoplasmic reticulum and in the Golgi apparatus of spermatids at around step 3. The antigen can further be found on the vesicles at thetrans-side of the Golgi apparatus, in the matrix of the head cap, and on the head cap membrane in step-4 to step-7 spermatids. The antigen appears to be concentrated at the equatorial segment during late spermiogenesis. Neither the (pro-)acrosomic granule nor the surrounding membrane are required in this pathway. This pathway can be termed the ‘Golgi-head cap tract’.
影响因子：
2.7
作者：
ARBOLEDA, CE;GERTON, GL
通讯作者：
GERTON, GL
影响因子：
3.6
作者：
ANAKWE, OO;GERTON, GL
通讯作者：
GERTON, GL