Heat-Transfer Resistance at Solid-Liquid Interfaces: A Tool for the Detection of Single-Nucleotide Polymorphisms in DNA
Heat-Transfer Resistance at Solid-Liquid Interfaces: A Tool for the Detection of Single-Nucleotide Polymorphisms in DNA
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DOI：
10.1021/nn300147e
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发表时间：
2012-03-01
期刊：
影响因子：
17.1
通讯作者：
Wagner, Patrick
中科院分区：
文献类型：
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作者：
van Grinsven, Bart;Vanden Bon, Natalie;Wagner, Patrick
在这篇文章中，我们报告了界面热阻作为一种新的基于变性的方法来检测DNA的单核苷酸多态。我们观察到，在人造金刚石表面接枝上的双链DNA分子刷并不显著地影响固-液界面的热阻。与此形成对比的是，令人惊讶的是，单链DNA的分子刷会导致更高的热阻，并发挥绝热层的作用。这一效应可以通过从低热阻到高热阻的切换来识别ds-DNA的熔融温度。用这种方法确定的不同DNA双链(29个碱基对，没有和有内置突变)的熔化温度与通过建模计算的数据很好地关联。该方法快速、无标记(不需要荧光或放射性标记)，允许重复测量，还可以扩展到阵列格式。通过共聚焦荧光显微镜和阻抗光谱的参考测量，证实了变性时热传递阻力的切换确实与DNA的热变性有关。
In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid Interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.