Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. I. Soluble agents.
Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. I. Soluble agents.
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用于体外标记吞噬白细胞的放射性试剂的调查。
DOI：
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发表时间：
1976
影响因子：
9.3
通讯作者：
M. Thakur
中科院分区：
文献类型：
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作者：
J. McAfee;M. Thakur
在存在或不存在血浆和红细胞的情况下，筛选了23种可溶性（非颗粒）放射性试剂在体外标记白细胞的能力。细胞结合的程度进行了比较与其他代理商以前报道。其中10种药物的白细胞结合率为添加放射性的2%或以上。高的标记产率，获得只有非极性脂溶性复合物能够穿透脂质细胞膜。不幸的是，这种类型的标记往往会被血浆蛋白减少。一些亲脂性放射性试剂在标记后倾向于从细胞中排出。与放射性颗粒相比，可溶性放射性药物提供了易于将细胞结合的活性与悬浮液中的残余活性离心分离的优点。然而，标记产率往往低于放射性颗粒，并且除了吞噬性白细胞之外，其他细胞成分也被标记。在本研究中，最有前途的可溶性试剂是8-羟基喹啉的111 In或99 mTc配合物、111 In-四苯基卟啉、放射性碘标记的DNA和77 Br标记的阿霉素配合物。任何这些药物的最终价值必须等待在实验动物和临床试验中进行充分的体内测试。
Twenty-three soluble (nonparticulate) radioactive agents were screened for their ability to label leukocytes in vitro in the presence or absence of plasma and red cells. The degree of cell binding was compared with that of other agents previously reported. Leukocyte binding of 2% or more of the added radioactivity was obtained with ten of these agents. High labeling yields were obtained only with nonpolar lipid-soluble complexes capable of penetrating lipid cell membranes. Unfortunately, this type of labeling tends to be reduced by plasma protein. Some lipophilic radioactive agents tend to elute from the cells after labeling. Compared with radioactive particles, soluble radiopharmaceuticals offer the advantage of easy centrifugal separation of the cell-bound from the residual activity in the suspension fluid. However, the labeling yield tends to be lower than for radioactive particles, and other cellular elements are labeled in addition to the phagocytic leukocytes. In this survey, the most promising soluble agents were 111In or 99mTc complexes of oxine, 111In-tetraphenylporphyrin, DNA labeled with radioactive iodine, and DNA labeled with 77Br and complexed with adriamycin. The ultimate value of any of these agents must await adequate in vivo testing in experimental animals and clinical trials.