Laboratory determinations in Anisakis simplex allergy
Laboratory determinations in Anisakis simplex allergy
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DOI：
10.1016/s0091-6749(96)80073-x
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发表时间：
1996-04-01
影响因子：
14.2
通讯作者：
Garcia, M
中科院分区：
文献类型：
--
作者：
delPozo, MD;Moneo, I;Garcia, M
背景：单纯异尖线虫引起的过敏反应目的：本研究旨在通过组胺释放试验，证实单纯异尖线虫能够触发IgE介导的过敏反应，并描述其致敏的血清学特征。方法：对12例进食熟鱼后出现过敏症状的患者，采用间接抗体夹心法和免疫印迹法检测单纯性异尖线虫过敏症状和IgE。被其他线虫寄生的受试者、鱼过敏患者和健康献血者的血清被诱导作为对照。结果：免疫印迹试验是检测单纯性弧菌变态反应的特异性试验。在11例患者的血清标本中发现IGE反应条带。酶联免疫吸附试验和免疫印迹试验均可检测到特异性抗体，但特异性较差。组胺释放在单纯沙打旺提取物中呈阳性反应，而与FISH阴性。结论：在我们的患者中发现了对单一沙棘提取物的特异性和强烈的免疫反应。单纯性阿司匹林可引起过敏反应，摄取鱼类后出现过敏症状的患者应怀疑为单纯性阿司匹林过敏。单纯性弧菌点刺试验阳性，FISH阴性是诊断单纯性弧菌过敏的良好指征。
Background: Anaphylactic reactions caused by the fish nematode, Anisakis simplObjective: This study was undertaken to confirm, by histamine release tests, that A. simplex is able to trigger IgE-mediated reactions and to describe the serologic profiles in this sensitization.Methods: Twelve patients who had anaphylactic symptoms after ingestion of cooked fish and positive prick test results and determinations of IgE to A. simplex were studied by indirect IgG ELISA and IgG and IgE immunoblotting. Sera from subjects parasitized with other nematodes, patients with fish allergy, and healthy donors were induced as controls. A histamine release test was performed in a representative case.Results: IgE immunoblotting was a specific test to detect A. simplex allergy. IgE-reacting bands were found in serum samples from 11 of our patients. Specific IgG antibodies were found by ELISA and immunoblotting, but this response was less specific. Histamine release was positive with A. simplex extract and negative with fish.Conclusion: A specific and intense immune response to an A. simplex extract was found in our patients. A. simplex is able to elicit anaphylactic reactions, and A. simplex allergy should be suspected in patients with allergic symptoms after ingestion of fish. A positive prick test response to A. simplex and negative response to fish is good indication for a diagnosis of A. simplex allergy.