Characteristics of new Staphylococcus aureus-RBC adhesion mechanism independent of fibrinogen and IgG under hydrodynamic shear conditions.
Characteristics of new Staphylococcus aureus-RBC adhesion mechanism independent of fibrinogen and IgG under hydrodynamic shear conditions.
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水动力剪切条件下独立于纤维蛋白原和 IgG 的新型金黄色葡萄球菌-红细胞粘附机制的特征。
DOI：
10.1152/ajpcell.00034.2005
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Ross,JuliaM
中科院分区：
文献类型：
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作者：
Shin,PyongKyun;Pawar,Parag;Konstantopoulos,Konstantinos;Ross,JuliaM
金黄色葡萄球菌感染开始于血液中循环的细菌细胞附着在宿主细胞外基质或内皮细胞的成分上并开始定植。金黄色葡萄球菌与血小板有广泛的相互作用。金黄色葡萄球菌在存在血浆蛋白（如纤维蛋白原和IgG）的情况下也会与红细胞结合。本文报道了一种新的s。金黄色红细胞独立于这些血浆蛋白。为了表征新的粘附机制，我们通过实验研究了ens之间的结合动力学和介导新的粘附相互作用的分子成分。在规定的剪切条件下，白细胞和红细胞。结果表明，s的纤维蛋白原（凝块因子A）和IgG（蛋白A）的受体。金黄色葡萄球菌不参与粘连。当剪切速率为100 s−1时，金黄色葡萄球菌与红细胞的粘附力最大，剪切速率越大，粘附力越弱。当剪切速率为2000 s−1时，剪切后形成的异质聚集体是稳定的，这表明在高剪切速率下，胞间接触时间而不是剪切力控制着黏附。金黄色葡萄球菌与红细胞的结合需要血浆，10%的血浆足以达到最大的粘附。参与细胞-细胞黏附的血浆蛋白，如纤维蛋白原、纤维连接蛋白、血管性血友病因子、IgG、血栓反应蛋白、层粘连蛋白和玻璃体粘连蛋白均未参与观察到的黏附。用胰蛋白酶、凝乳胰蛋白酶或神经氨酸酶处理红细胞时，异聚集的程度显著降低，这表明介导异聚集过程的受体是红细胞表面唾液化的糖蛋白。粘附依赖于二价阳离子，也可被肝素阻断。这项工作证明了s的一种新的机制。在流体动力剪切条件下，通过未知的红细胞唾液糖蛋白与金黄色葡萄球菌结合。这种结合需要血浆蛋白(s)，而不是纤维蛋白原或IgG，不涉及这些。金黄色粘连素凝集因子A或蛋白A。
Staphylococcus aureusinfection begins when bacterial cells circulating in blood adhere to components of the extracellular matrix or endothelial cells of the host and initiate colonization.S. aureusis known to exhibit extensive interactions with platelets.S. aureusis also known to bind to red blood cells (RBCs) in the presence of plasma proteins, such as fibrinogen and IgG. Herein we report a new binding mechanism ofS. aureusto RBC independent of those plasma proteins. To characterize the new adhesion mechanism, we experimentally examine the binding kinetics and molecular constituents mediating the new adhesive interactions betweenS. aureusand RBCs under defined shear conditions. The results demonstrate that the receptors for fibrinogen (clumping factor A) and IgG (protein A) ofS. aureusare not involved in the adhesion.S. aureusbinds to RBCs with maximal adhesion at the shear rate 100 s−1and decreasing adhesion with increasing shear. The heteroaggregates formed after shear are stable when subjected to the shear rate 2,000 s−1, indicating that intercellular contact time rather than shear forces controls the adhesion at high shear.S. aureusbinding to RBC requires plasma, and 10% plasma is sufficient for maximal adhesion. Plasma proteins involved in the cell-cell adhesion, such as fibrinogen, fibronectin, von Willebrand factor, IgG, thrombospondin, laminin, and vitronectin are not involved in the observed adhesion. The extent of heteroaggregation is dramatically reduced on RBC treatment with trypsin, chymotrypsin, or neuraminidase, suggesting that the receptor(s) mediating the heteroaggregation process is a sialylated glycoprotein on RBC surface. Adhesion is divalent cation dependent and also blocked by heparin. This work demonstrates a new mechanism ofS. aureus-RBC binding under hydrodynamic shear conditions via unknown RBC sialoglycoprotein(s). The binding requires plasma protein(s) other than fibrinogen or IgG and does not involve theS. aureusadhesins clumping factor A or protein A.