Transposon-independent increase of transcription by the Sleeping Beauty transposase
Transposon-independent increase of transcription by the Sleeping Beauty transposase
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DOI：
10.1016/j.bbrc.2004.03.116
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发表时间：
2004-05-07
影响因子：
3.1
通讯作者：
Kaneda, Y
中科院分区：
文献类型：
--
作者：
Masuda, K;Yamamoto, S;Kaneda, Y
将编码睡美人（SB）转座酶的质粒（pCMV-SB）与荧光素酶表达质粒DNA以4：1的摩尔比共导入小鼠骨骼肌中。在第28天，荧光素酶基因的表达比没有pCMV-SB的表达高5倍。这种增强作用不依赖于荧光素酶表达质粒中转座子（Tn）序列的存在。Southern印迹分析未能检测到10个荧光素酶基因插入到宿主基因组中。将不含Tn的荧光素酶表达质粒共导入有或无pCMV-SB的HcLa细胞中进行表达，pCMV-SB的mRNA表达量和荧光素酶活性分别提高了1.5倍和2倍。结果表明，SB转座酶能增强外源基因的转录，而与Tn序列无关。(C)2004年爱思唯尔公司All rights reserved.
When a plasmid encoding the Sleeping Beauty (SB) transposase (pCMV-SB) was cointroduced with luciferase expression plasmid DNA into Mouse skeletal muscle at a molar ratio of 4:1. luciferase gene expression was 5-times higher than the expression Without pCMV-SB on day 28. This enhancement was not dependent on the presence of transposon (Tn) sequence in luciferase expression plasmid. southern blot analysis failed 10 detect luciferase gene insertion into the host genome. Then, expression, a luciferase expression plasmid without Tn was cointroduced into HcLa cells with or without pCMV-SB. With pCMV-SB, the mRNA amount and the luciferase activity was 1.5 times and 2 times higher. respectively, than without pCMV-SB, even though the cells with pCMV-SB had a smaller copy number of luciferase plasmid than the cells without pCMV-SB. These results Suggest that SB transposase enhances the transcription of an exogenous gene regardless of the presence of the Tn sequence. (C) 2004 Elsevier Inc. All rights reserved.