RELATING BIOCHEMISTRY TO BIOLOGY - HOW THE RECOMBINATIONAL REPAIR FUNCTION OF RECA PROTEIN IS MANIFESTED IN ITS MOLECULAR-PROPERTIES
RELATING BIOCHEMISTRY TO BIOLOGY - HOW THE RECOMBINATIONAL REPAIR FUNCTION OF RECA PROTEIN IS MANIFESTED IN ITS MOLECULAR-PROPERTIES
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DOI：
10.1002/bies.950150908
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发表时间：
1993-09-01
期刊：
影响因子：
4
通讯作者：
COX, MM
中科院分区：
文献类型：
--
作者：
COX, MM
RecA蛋白在DNA代谢中的多种活性激发了世界各地数十个实验室十多年的研究。然而，这一努力未能产生几个RecA蛋白介导的过程，其中DNA链交换反应突出的机制的理解。阻碍进展的主要因素是无效的限制放在问题上，试图了解RecA蛋白介导的DNA链交换的背景下，一个不适当的生物学范式-即同源遗传重组作为一种机制，产生遗传多样性。在这篇文章中，我总结的遗传和生化数据表明，RecA蛋白进化的重组DNA修复系统的核心组成部分，与遗传多样性的产生是一个有时有用的副产品，并审查RecA蛋白的主要体外活动从修复的角度。虽然提出的重组和重组修复模型通常使用看起来非常相似的DNA链切割和转移步骤，但这两个过程的分子和热力学要求非常不同。重组修复功能为思考RecA的生化特性及其促进的链交换反应提供了一个更具逻辑性和信息性的框架。
The multiple activities of the RecA protein in DNA metabolism have inspired over a decade of research in dozens of laboratories around the world. This effort has nevertheless failed to yield an understanding of the mechanism of several RecA protein-mediated processes, the DNA strand exchange reactions prominent among them. The major factors impeding progress are the invalid constraints placed upon the problem by attempting to understand RecA protein-mediated DNA strand exchange within the context of an inappropriate biological paradigm - namely, homologous genetic recombination as a mechanism for generating genetic diversity. In this essay I summarize genetic and biochemical data demonstrating that RecA protein evolved as the central component of a recombinational DNA repair system, with the generation of genetic diversity being a sometimes useful byproduct, and review the major in vitro activities of RecA protein from a repair perspective. While models proposed for both recombination and recombinational repair often make use of DNA strand cleavage and transfer steps that appear to be quite similar, the molecular and thermodynamic requirements of the two processes are very different. The recombinational repair function provides a much more logical and informative framework for thinking about the biochemical properties of RecA and the strand exchange reactions it facilitates.