Localization of stereotaxic coordinates for the ventral tegmental area in early adolescent, mid-adolescent and adult rats.
Localization of stereotaxic coordinates for the ventral tegmental area in early adolescent, mid-adolescent and adult rats.
复制标题
青春期早期、青春期中期和成年大鼠腹侧被盖区立体定位坐标的定位。
DOI：
10.1016/j.brainres.2008.04.060
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发表时间：
2008
期刊：
影响因子：
2.9
通讯作者：
Kirstein,CherylL
中科院分区：
文献类型：
--
作者：
Sponaugle,AshleyE;Badanich,KimberlyA;Kirstein,CherylL
腹侧被盖区（VTA）是一个与药物成瘾和相关行为有关的脑区，然而，很少有研究探讨VTA在青春期大鼠这些过程中的作用。了解VTA的发展对于阐明青少年对药物成瘾的脆弱性的介导机制至关重要。本研究的目的是确定发育中大鼠腹侧被盖区的立体定位坐标。在青春期早期[出生后（PND）28天]、青春期中期（PND 35天）或成年雄性大鼠（PND 70天）手术植入导向套管，对准腹侧被盖区。成人坐标，（P：−3.5，L：+1.0，V：-8.5 mm）作为基线，并指导青少年早期和中期大鼠的VTA坐标定位。恢复后，通过微透析探针注射染料，取出脑，切片并染色，用于VTA中插管放置的组织学验证。数据表明青少年的VTA坐标与成年大鼠有显著差异。这些研究结果意味着，它是必须考虑的VTA的发展解剖学差异时，比较滥用药物在青少年和成年大鼠的神经化学作用。
The ventral tegmental area (VTA) is a brain region implicated in drug addiction and related behaviors; however, little research has been conducted examining the role of the VTA in these processes in adolescent rats. Understanding the development of the VTA is imperative for elucidating mechanisms mediating adolescent vulnerability to drug addiction. The purpose of the present study was to define stereotaxic coordinates for the VTA in developing rats. Early adolescent [postnatal day (PND) 28], mid-adolescent (PND 35), or adult male rats (PND 70) were surgically implanted with a guide cannula aimed at the VTA. Adult coordinates, (P: −3.5, L: +1.0, V: −8.5 mm from Bregma) were used as a baseline and guided localization of VTA coordinates for early and mid-adolescent rats. After recovery, dye was injected via a microdialysis probe and brains were removed, sliced, and stained for histological verification of cannula placement in the VTA. Data suggest VTA coordinates in adolescents differ significantly from adult rats. These findings imply that it is imperative to consider anatomical differences in the development of the VTA when comparing the neurochemical effects of abused drugs in adolescent and adult rats.