Effects of smoking on activation markers, Fas expression and apoptosis of peripheral blood lymphocytes
Effects of smoking on activation markers, Fas expression and apoptosis of peripheral blood lymphocytes
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DOI：
10.1046/j.1365-2362.2001.00842.x
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发表时间：
2001-06-01
影响因子：
5.5
通讯作者：
Kallenberg, CGM
中科院分区：
文献类型：
--
作者：
Bijl, M;Horst, G;Kallenberg, CGM
背景吸烟影响外周血淋巴细胞（PBL）的数量和功能，其影响机制尚不清楚。我们进行了这项研究，以评估是否吸烟的免疫损伤可能与Fas的表达或功能的变化，细胞表面分子，发挥了核心作用，在免疫稳态和细胞毒activity.Methods PBL从10吸烟和10非吸烟健康志愿者分离。流式细胞术检测PBL的活化状态、Fas表达和凋亡。Fas的功能休息后，通过评估细胞凋亡的激动性抗Fas抗体在4个吸烟和4个nonsmokingindividual.Results吸烟与Fas表达的CD4（+）T和B淋巴细胞的百分比增加。观察到活化（CD 38（+））B细胞百分比降低。在体外Fas诱导的细胞凋亡没有出现吸烟者和非吸烟者之间的差异。结论吸烟与外周血淋巴细胞Fas表达增加有关，尤其是B细胞Fas表达增加。这可能使这些细胞更容易发生凋亡。由于Fas在功能上是完整的，这也可以解释在吸烟个体中发现的活化（CD 38（+））B细胞的百分比降低。后者可能有助于降低体液免疫反应中观察到的吸烟者。
Background Smoking influences numbers and function of peripheral blood lymphocytes (PBL) by a process that is badly understood. We conducted this study to evaluate whether the immune impairment of smoking might be related to changes in the expression or functionality of Fas, a cell surface molecule that plays a central role in immune homeostasis and cytotoxic activity.Methods PBL from 10 smoking and 10 nonsmoking healthy volunteers were isolated. Flow cytometry was performed to measure the state of activation, Fas expression and apoptosis of PBL. Functionality of Fas was rested by assessing apoptosis after incubation of isolated lymphocytes with agonistic anti-Fas antibodies in four smoking and four nonsmoking individuals.Results Smoking was associated with an increase in the percentage of Fas-expressing CD4(+) T and B lymphocytes. A decrease in the percentage of activated (CD38(+)) B cells was observed. In vitro Fas-induced apoptosis did not appear different between smokers and nonsmokers. No differences in the percentages of circulating apoptotic lymphocytes could be demonstrated between smoking and nonsmoking individuals.Conclusion Smoking is associated with increased Fas expression on PBL in general, and on B cells in particular. This might render these cells more susceptible for apoptosis. As Fas is functionally intact this may also explain the reduced percentage of activated (CD38(+)) B cells found in smoking individuals. The latter may contribute to the reduced humoral immune response observed in smokers.