Octacalcium phosphate combined with collagen orthotopically enhances bone regeneration
Octacalcium phosphate combined with collagen orthotopically enhances bone regeneration
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DOI：
10.1002/jbm.b.30531
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发表时间：
2006-11-01
影响因子：
3.4
通讯作者：
Suzuki, Osamu
中科院分区：
文献类型：
--
作者：
Kamakura, Shinji;Sasaki, Kazuo;Suzuki, Osamu
磷酸八钙（OCP）是一种可吸收的骨再生材料，但其脆性使其难以无约束地保持其形状。我们设计了一种由合成的OCP和猪胶原海绵（OCP/胶原）构建的支架，并研究了OCP/胶原复合物是否可以促进骨再生。为了验证这一假设，将OCP/胶原植入的骨再生与OCP和胶原植入的骨再生进行了比较。进行放射学和组织学检查，并通过组织形态计量学分析确定缺损处新形成骨的百分比（n-Bone%）。将OCP/Coliagen、OCP或胶原植入大鼠颅骨中的临界大小的缺损中，并在植入后2、4或8周固定。OCP/Collagen比OCP颗粒提高了操作性能，并且协同增强了骨再生，这是由OCP的骨成核和胶原的细胞浸润组成的。组织形态计量学分析显示，在第8周，OCP/胶原蛋白（48.4 +/- 5.14）治疗的n-骨% +/-标准误显著高于OCP（27.6 +/- 4.04）或胶原蛋白（27.4 +/- 5.69）治疗的n-骨% +/-标准误。本研究表明，OCP与胶原蛋白的组合可产生骨再生的协同作用。(c)2006 Wiley Periodicals，Inc.
Octacalcium phosphate (OCP) is resorbable bone regenerative material, but its brittleness makes it difficult to maintain its shape without restraint. We have engineered a scaffold constructed of synthetic OCP and porcine collagen sponge (OCP/Collagen) and investigated whether OCP/Collagen composite could improve bone regeneration. To examine this hypothesis, bone regeneration by the implantation of OCP/Collagen was compared with those by OCP and collagen. Radiographic and histological examination was performed and the percentage of newly formed bone (n-Bone%) in the defect was determined by a histomorphometrical analysis. OCP/Coliagen, OCP, or collagen was implanted into the critical-sized defects in rat crania and fixed at 2, 4, or 8 weeks after implantation. OCP/Collagen improved the handling performance than the granules of OCP, and synergistically enhanced the bone regeneration beyond expectation, which were composed of bone nucleation by OCP and cell infiltration by collagen. Histomorphometrical analysis showed that n-Bone% +/- standard error treated with OCP/Collagen (48.4 +/- 5.14) was significantly higher than those with OCP (27.6 +/- 4.04) or collagen (27.4 +/- 5.69) in week 8. The present study suggests that the combination OCP with collagen elicited the synergistic effect for bone regeneration. (c) 2006 Wiley Periodicals, Inc.