THE COAT PROTEIN OF BEET CURLY TOP VIRUS IS ESSENTIAL FOR INFECTIVITY
THE COAT PROTEIN OF BEET CURLY TOP VIRUS IS ESSENTIAL FOR INFECTIVITY
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DOI：
10.1016/0042-6822(89)90205-5
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发表时间：
1989-10-01
期刊：
影响因子：
3.7
通讯作者：
STANLEY, J
中科院分区：
文献类型：
--
作者：
BRIDDON, RW;WATTS, J;STANLEY, J
我们已经申请的程序，农杆菌介导的接种，以开发一个简单的，有效的，和重复性的测定叶蝉传播的双生病毒，甜菜曲顶病毒（BTV）的感染性。该测定系统用于显示BCTV的外壳蛋白突变体不具有感染性，但是可以通过与在互补正义开放阅读框中具有致死突变的第二突变体C1共接种来补充。此外，外壳蛋白突变体保留了复制和产生ssDNA和dsDNA的能力，当电穿孔到烟草原生质体。我们的结论是，外壳蛋白的BCTV是必不可少的传播病毒。结果进行了讨论，根据与其他生殖病毒的外壳蛋白突变体的结果。
We have applied the procedure of Agrobacterium-mediated inoculation to develop a simple, efficient, and reproducible assay for the infectivity of the leafhopper-transmitted geminivirus, beet curly top virus (BCTV). This assay system was used to show that a coat protein mutant of BCTV is not infectious, but could be complemented by coagroinoculation with a second mutant bearing a lethal mutation in the complementary-sense open reading frame, C1. Furthermore, the coat protein mutant retained the ability to replicate and to produce both ssDNA and dsDNA when electroporated into Nicotiana tabacum protoplasts. We conclude that the coat protein of BCTV is essential for spread of the virus. The results are discussed in the light of results with coat protein mutants of other germiniviruses.