Gold silver alloy nanoparticles (GSAN): an imaging probe for breast cancer screening with dual-energy mammography or computed tomography.
Gold silver alloy nanoparticles (GSAN): an imaging probe for breast cancer screening with dual-energy mammography or computed tomography.
复制标题
DOI：
10.1039/c6nr02618d
复制
发表时间：
2016-07-14
期刊：
影响因子：
6.7
通讯作者：
Cormode DP
中科院分区：
文献类型：
--
作者：
Naha PC;Lau KC;Hsu JC;Hajfathalian M;Mian S;Chhour P;Uppuluri L;McDonald ES;Maidment AD;Cormode DP
及早发现乳腺癌可降低该疾病的死亡率。因此，开发更好的筛选技术成为人们强烈关注的话题。对比增强双能乳房X线摄影（DEM）是一种新技术，可以提高癌症检测的灵敏度。然而，用于该技术的造影剂的开发还处于起步阶段。我们在此报告了金银合金纳米颗粒 (GSAN)，它具有强大的 DEM 对比度特性和改善的生物相容性。使用各种分析方法合成并表征了含有一系列金银比例并用 m-PEG 封端的 GSAN 制剂。 DEM 和计算机断层扫描 (CT) 幻像成像表明，GSAN 产生的对比度与单独的银相当。细胞活力、活性氧生成和DNA损伤结果表明，金含量为30%或更高的制剂与Hep G2和J774A.1细胞具有细胞相容性。在有和没有乳腺肿瘤的小鼠中进行体内成像。结果表明，GSAN产生强烈的DEM和CT对比并在肿瘤中积累。此外，体内成像和离体分析均表明 GSAN 通过尿液和粪便排泄。总之，GSAN 产生强大的 DEM 和 CT 对比度，并且具有用于血池成像和乳腺癌筛查的潜力。
Earlier detection of breast cancer reduces mortality from this disease. As a result, the development of better screening techniques is a topic of intense interest. Contrast-enhanced dual-energy mammography (DEM) is a novel technique that has improved sensitivity for cancer detection. However, the development of contrast agents for this technique is in its infancy. We herein report gold-silver alloy nanoparticles (GSAN) that have potent DEM contrast properties and improved biocompatibility. GSAN formulations containing a range of gold:silver ratios and capped with m-PEG were synthesized and characterized using various analytical methods. DEM and computed tomography (CT) phantom imaging showed that GSAN produced robust contrast that was comparable to silver alone. Cell viability, reactive oxygen species generation and DNA damage results revealed that the formulations with 30% or higher gold content are cytocompatible to Hep G2 and J774A.1 cells. In vivo imaging was performed in mice with and without breast tumors. The results showed that GSAN produce strong DEM and CT contrast and accumulated in tumors. Furthermore, both in vivo imaging and ex vivo analysis indicated the excretion of GSAN via both urine and feces. In summary, GSAN produce strong DEM and CT contrast, and has potential for both blood pool imaging and for breast cancer screening.