Somatic gene transfer of NGF to the aged brain: behavioral and morphological amelioration
Somatic gene transfer of NGF to the aged brain: behavioral and morphological amelioration
复制标题
NGF 体细胞基因转移至衰老大脑：行为和形态改善
作者：
Ks Chen;F. Gage
将分泌神经生长因子（NGF）的原代成纤维细胞移植到老年记忆受损大鼠大细胞基底核（NBM）。产生NGF的成纤维细胞在移植后存活了6周，并在整个实验期间持续表达NGF mRNA。结果表明，大鼠的记忆功能明显改善，基底前脑低亲和力NGF受体（p75）阳性神经元的大小和数量明显增加。将产生NGF的细胞植入正常的年轻成年大鼠体内，导致短暂但明显的记忆障碍和低亲和力NGF受体阳性神经元的肥大。这些结果表明，自然发生的与年龄相关的记忆丧失可以通过直接向NBM移植分泌NGF的细胞来逆转，并且胆碱能过度或功能减退都可能导致认知障碍。
Primary fibroblasts modified to secrete nerve growth factor (NGF) were implanted into the nucleus basalis magnocellularis (NBM) of aged memory impaired rats. The NGF-producing fibroblasts survived for 6 weeks following transplantation and continued expressing NGF mRNA through the duration of the experiment. A significant amelioration of the memory impairment and a significant increase in size and number of low- affinity NGF receptor (p75)-positive neurons in the basal forebrain were observed. Implantation of NGF-producing cells into normal young adult rats resulted in a transient but significant memory impairment and hypertrophy of low-affinity NGF receptor-positive neurons. These results show that naturally occurring age-related memory loss can be reversed by grafting cells engineered to secrete NGF directly to the NBM, and that either cholinergic hyper- or hypofunction may lead to cognitive impairments.
将转基因细胞移植到受损的大脑：NGF 表达的恢复作用。
DOI：
10.1126/science.3201248
发表时间：
1988
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rosenberg,MB;Friedmann,T;Robertson,RC;Tuszynski,M;Wolff,JA;Breakefield,XO;Gage,FH
通讯作者：
Gage,FH