Isolation and biochemical characterization of a new NADH oxidase from Lactobacillus brevis
Isolation and biochemical characterization of a new NADH oxidase from Lactobacillus brevis
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DOI：
10.1023/a:1021730131633
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发表时间：
2003-01-01
影响因子：
2.7
通讯作者：
Riebel, B
中科院分区：
文献类型：
--
作者：
Hummel, W;Riebel, B
从短链乳杆菌中分离并鉴定了一种新的NADH氧化酶，可用于NAD+的再生。粗提物的活性在10-15U mg(-1)之间。经四步层析纯化后，活性为116U mg(-1)，产率为14%。酶活最高的温度为40℃，最适pH为5.5-7。这种酶需要二硫苏糖醇来防止氧化失活。NADH的K-m值为24微米。
A new NADH oxidase, useful for the regeneration of NAD+, was isolated and characterized from Lactobacillus brevis. In crude extracts the activity was from 10-15 U mg(-1). After purification by four chromatographic steps, an activity of 116 U mg(-1) was obtained with 14% yield. Highest activity was from pH 5.5-7 and at 40degreesC. The enzyme requires dithiothreitol to prevent oxidative deactivation. The K-m value for NADH was 24 muM.