Root-knot nematodes modulate cell walls during root-knot formation in Arabidopsis roots.
Root-knot nematodes modulate cell walls during root-knot formation in Arabidopsis roots.
复制标题
根结线虫在拟南芥根的根结形成过程中调节细胞壁。
DOI：
10.1007/s10265-020-01186-z
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
S.
中科院分区：
文献类型：
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作者：
Ishida;T.;Suzuki;R.;Nakagami;S.;Kuroha;T.;Sakamoto;S.;Nakata;M.T.;Yokoyama;R.;Kimura;S.;Mitsuda;N.;Nishitani;K.;Sawa;S.
植物寄生线虫寄生于多种生根植物中以吸收养分，从而导致寄主植物出现严重的生长缺陷。在感染过程中，根结线虫会诱导宿主植物根部形成一种称为根结或瘿的特征性增生结构。尽管许多先前的研究解决了这种异常的形态发生，但其潜在机制仍然未知。为了在分子水平上分析植物与微生物的相互作用，我们利用南方根结线虫和模式植物拟南芥建立了体外感染测定系统。时程 mRNA-seq 分析表明，瘿中原形成层相关基因的水平增加，表明血管干细胞在瘿形成中发挥重要作用。相反，虫瘿中参与次生细胞壁形成的基因减少。中性糖分析表明，木聚糖（主要次生细胞壁成分之一）的水平在虫瘿中显着降低。这些观察结果与高度分化细胞数量减少和未分化细胞密度增加导致胆瘿形成的假设一致。我们的研究结果表明，植物寄生线虫调节宿主的发育机制，以改变植物生理过程的各个方面并建立取食场所。
Phytoparasitic nematodes parasitize many species of rooting plants to take up nutrients, thus causing severe growth defects in the host plants. During infection, root-knot nematodes induce the formation of a characteristic hyperplastic structure called a root-knot or gall on the roots of host plants. Although many previous studies addressed this abnormal morphogenesis, the underlying mechanisms remain uncharacterized. To analyze the plant–microorganism interaction at the molecular level, we established an in vitro infection assay system using the nematodeMeloidogyne incognitaand the model plantArabidopsis thaliana. Time-course mRNA-seq analyses indicated the increased levels of procambium-associated genes in the galls, suggesting that vascular stem cells play important roles in the gall formation. Conversely, genes involved in the formation of secondary cell walls were decreased in galls. A neutral sugar analysis indicated that the level of xylan, which is one of the major secondary cell wall components, was dramatically reduced in the galls. These observations were consistent with the hypothesis of a decrease in the number of highly differentiated cells and an increase in the density of undifferentiated cells lead to gall formation. Our findings suggest that phytoparasitic nematodes modulate the developmental mechanisms of the host to modify various aspects of plant physiological processes and establish a feeding site.