In situ analyses of genome instability in breast cancer
In situ analyses of genome instability in breast cancer
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DOI：
10.1038/ng1409
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发表时间：
2004-09-01
期刊：
影响因子：
30.8
通讯作者：
Lockett, SJ
中科院分区：
文献类型：
--
作者：
Chin, K;de Solorzano, CO;Lockett, SJ
端粒危机的转变被认为是大多数乳腺癌发展的关键事件。我们在这项研究中的目标是确定在组织学定义的乳腺癌进展的背景下，这种情况发生在哪里。为此，我们评估了基因组不稳定性（使用荧光原位杂交）以及与正常导管上皮、常见导管增生、导管原位癌和浸润性癌中端粒危机相关的其他特征。我们通过测量人类乳腺上皮细胞培养物在 ZNF217 介导的端粒危机和永生化转变过程中的这些相同特征，在体外模拟了这一过程。总而言之，数据表明乳腺癌中端粒危机和永生化的转变发生在从普通导管增生到导管原位癌的进展过程中。
Transition through telomere crisis is thought to be a crucial event in the development of most breast carcinomas. Our goal in this study was to determine where this occurs in the context of histologically defined breast cancer progression. To this end, we assessed genome instability (using fluorescence in situ hybridization) and other features associated with telomere crisis in normal ductal epithelium, usual ductal hyperplasia, ductal carcinoma in situ and invasive cancer. We modeled this process in vitro by measuring these same features in human mammary epithelial cell cultures during ZNF217-mediated transition through telomere crisis and immortalization. Taken together, the data suggest that transition through telomere crisis and immortalization in breast cancer occurs during progression from usual ductal hyperplasia to ductal carcinoma in situ.