Genomic screening for Chlamydophila pneumoniae-specific antigens using serum samples from patients with primary infection
Genomic screening for Chlamydophila pneumoniae-specific antigens using serum samples from patients with primary infection
复制标题
使用原发感染患者的血清样本对肺炎衣原体特异性抗原进行基因组筛查
DOI：
10.1111/j.1574-6968.2012.02520.x
复制
发表时间：
2012
影响因子：
2.1
通讯作者：
Kishi F
中科院分区：
文献类型：
--
作者：
Yasui Y;Yanatori I;Kawai Y;Miura K;Suminami Y;Hirota T;Tamari M;Ouchi K;Kishi F
嗜肺炎衣原体是一种专性细胞内人类病原体，可引起呼吸道感染。C.肺炎衣原体感染的检测方法有微量免疫荧光试验和商业血清学ELISA试验;这些试验分别基于检测针对完整衣原体原体和脂多糖/外膜蛋白的抗体。鉴定更特异和高度免疫显性的抗原对于开发新的血清学诊断检测是必不可少的。目的：寻找新的C. pneumoniae中，筛选出455个功能未知的基因。肺炎J138.表达GFP标记的酵母细胞提取物C.肺炎克雷伯氏菌的血清样品进行蛋白质印迹分析。肺炎患者作为一级抗体。从这个综合分析，58个克隆表达C.肺炎开放阅读框，包括假设的蛋白质，被鉴定为抗原。这些结果为开发新的C.肺炎感染和未来疫苗的开发。
Chlamydophila pneumoniae, an obligate intracellular human pathogen, causes respiratory tract infections. The most common techniques used for the serological diagnosis ofC. pneumoniaeinfections are microimmunofluorescence tests and commercial serological ELISA tests; these are based on the detection of antibodies against whole chlamydial elementary bodies and lipopolysaccharide/outer membrane protein, respectively. Identification of more specific and highly immunodominant antigens is essential for the development of new serodiagnostic assays. To identify novel specific antigens fromC. pneumoniae, we screened 455 genes with unknown function in the genome ofC. pneumoniaeJ138. Extracts ofSaccharomyces cerevisiaecells expressing GFP-taggedC. pneumoniaeproteins were subjected to Western blot analysis using serum samples fromC. pneumoniae-infected patients as the primary antibodies. From this comprehensive analysis, 58 clones expressingC. pneumoniaeopen reading frames, including hypothetical proteins, were identified as antigens. These results have provided useful information for the development of new serological tools for the diagnosis forC. pneumoniaeinfections and for the development of vaccines in future.