Mammalian interspecies substitution of immune modulatory alleles by genome editing.
Mammalian interspecies substitution of immune modulatory alleles by genome editing.
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DOI：
10.1038/srep21645
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发表时间：
2016-02-22
影响因子：
4.6
通讯作者：
Whitelaw CB
中科院分区：
文献类型：
--
作者：
Lillico SG;Proudfoot C;King TJ;Tan W;Zhang L;Mardjuki R;Paschon DE;Rebar EJ;Urnov FD;Mileham AJ;McLaren DG;Whitelaw CB
我们描述了动物基因工程的一个根本性的新壮举：将农艺单倍型精确而有效地替换成驯化的物种。RELA基因座的胚胎内锌指核酸酶编辑产生了具有与非洲猪瘟恢复力相关的疣猪RELA直系同源物的活产家猪。在一代中有效实现种间等位基因渐渗的能力为农业和基础研究提供了前所未有的机会。
We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated with resilience to African Swine Fever. The ability to efficiently achieve interspecies allele introgression in one generation opens unprecedented opportunities for agriculture and basic research.